Warren R L, Baker N R, Johnson J, Stapleton M J
Antimicrob Agents Chemother. 1985 Apr;27(4):468-72. doi: 10.1128/AAC.27.4.468.
Gentamicin and tobramycin inhibited the accumulation of extracellular proteases secreted by Pseudomonas aeruginosa. The secretion of protease was inhibited at concentrations of these drugs that were below the level required to inhibit general protein synthesis. Neither magnesium ions nor high ionic strength antagonized the ability of the aminoglycosides to block secretion of the proteases. Under these culture conditions magnesium ions were shown to antagonize the effects of the aminoglycosides on protein synthesis and aminoglycoside-mediated lysozyme lysis of P. aeruginosa. These results suggested that the drugs blocked secretion of the proteases by acting at the level of the outer membrane.
庆大霉素和妥布霉素可抑制铜绿假单胞菌分泌的细胞外蛋白酶的积累。在这些药物浓度低于抑制一般蛋白质合成所需水平时，蛋白酶的分泌就受到了抑制。镁离子和高离子强度均未拮抗氨基糖苷类药物阻断蛋白酶分泌的能力。在这些培养条件下，镁离子可拮抗氨基糖苷类药物对蛋白质合成的影响以及氨基糖苷类药物介导的铜绿假单胞菌溶菌酶裂解作用。这些结果表明，这些药物通过在外膜水平发挥作用来阻断蛋白酶的分泌。
