Lohr D, Van Holde K E
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6326-30. doi: 10.1073/pnas.76.12.6326.
Detailed analysis of the DNA fragment patterns produced by DNase I digestion of yeast, HeLa, and chicken erythrocyte nuclei reveals surprising features of nucleosome phasing. First, the spacer regions in phased yeast chromatin must be of lengths (10m + 5) base pairs, where m = 0, 1, 2,.... This feature is not seen in parallel studies of chicken erythrocyte chromatin. The 5-base pair increment in the yeast spacer imposes interesting restraints on the higher order structure of yeast chromatin. Second, we have been able to simulate the DNase I cutting patterns and get good agreement with the observed yeast patterns. Third, three different chromatins show a long range periodicity in the DNase I digest pattern, with a period half that of the staphylococcal nuclease repeat. These results suggest that the amount of chromatin observed in discrete extended-ladder bands is a minimum estimate of phasing and in fact phasing may be a more general feature.
对经脱氧核糖核酸酶I消化酵母、海拉细胞和鸡红细胞核所产生的DNA片段模式进行的详细分析揭示了核小体相位的惊人特征。首先，相位化酵母染色质中的间隔区长度必须为(10m + 5)个碱基对，其中m = 0、1、2 等等。在对鸡红细胞染色质的平行研究中未观察到这一特征。酵母间隔区中5个碱基对的增量对酵母染色质的高级结构施加了有趣的限制。其次，我们能够模拟脱氧核糖核酸酶I的切割模式，并与观察到的酵母模式取得良好的一致性。第三，三种不同的染色质在脱氧核糖核酸酶I消化模式中显示出长程周期性，其周期是葡萄球菌核酸酶重复周期的一半。这些结果表明，在离散的延伸梯状条带中观察到的染色质数量是相位化的最低估计值，实际上相位化可能是一个更普遍的特征。
