Daher Malek G, Taya Sofyan A, Colak Ilhami, Patel Shobhit K, Olaimat Melad M, Ramahi Omar
Department of Physics, Islamic University of Gaza, Gaza, Palestine.
Department of Electrical and Computer Engineering, University of Waterloo, Waterloo, Ontario, Canada.
J Biophotonics. 2022 May;15(5):e202200001. doi: 10.1002/jbio.202200001. Epub 2022 Feb 7.
As a result of the risks that waterborne bacteria bring to the human body, identifying them in drinking water has become a global concern. In this article, a highly sensitive surface plasmon resonance (SPR) biosensor consisting of prism, Ag, graphene, affinity layer and sensing medium is proposed for rapid detection of the waterborne bacteria. Four SPR-based sensors are first studied with the structures prism/Ag/sensing medium, prism/Ag/affinity layer/sensing medium, prism/Ag/graphene/sensing medium, and prism/Ag/graphene/affinity layer/sensing medium. The latter structure is found to have the highest sensitivity so it is considered for further investigations. Four different commonly used prisms are then demonstrated which are N-FK51A, 2S2G, SF10 and BK7. The structure with the prism N-FK51A is found to correspond to the highest sensitivity so it is considered for further investigations. The structure parameters are then optimized. The proposed SPR sensor can achieve high sensitivity of about 221.63 °/RIU for Escherichia coli and 178.12 °/RIU for Vibrio cholera bacteria with an average value of 199.87 °/RIU. We believe that the proposed structure will open a new window in the field of microorganism detections.
由于水传播细菌给人体带来的风险，在饮用水中识别它们已成为全球关注的问题。在本文中，提出了一种由棱镜、银、石墨烯、亲和层和传感介质组成的高灵敏度表面等离子体共振（SPR）生物传感器，用于快速检测水传播细菌。首先研究了四种基于SPR的传感器，其结构分别为棱镜/银/传感介质、棱镜/银/亲和层/传感介质、棱镜/银/石墨烯/传感介质和棱镜/银/石墨烯/亲和层/传感介质。发现后一种结构具有最高的灵敏度，因此考虑对其进行进一步研究。然后展示了四种不同的常用棱镜，即N-FK51A、2S2G、SF10和BK7。发现采用棱镜N-FK51A的结构具有最高的灵敏度，因此考虑对其进行进一步研究。随后对结构参数进行了优化。所提出的SPR传感器对大肠杆菌可实现约221.63°/RIU的高灵敏度，对霍乱弧菌可实现178.12°/RIU的高灵敏度，平均值为199.87°/RIU。我们相信，所提出的结构将为微生物检测领域打开一扇新的窗口。
