McMenamin C, Jarrett E E, Sanderson A
Immunology. 1985 Jul;55(3):399-403.
We have previously shown that lymphocytes from Nippostrongylus brasiliensis infected rats, when stimulated with antigen or concanavalin A (Con A) release factors which are comparable with murine IL-3. On addition of these factors to rat bone marrow cultures, mast cells with the morphological and biochemical properties of mucosal mast cells (MMC) proliferate and mature. Here, we use this system, along with monoclonal antibodies against rat T cells and the fluorescence-activated cell sorter (FACS), to isolate the subset of T cells responsible for the production of this MMC growth factor. Lymphocytes from N. brasiliensis infected rats were separated on the FACs into populations with and without the antigens defined by OX19, W3/25 and OX8 monoclonal antibodies; these antibodies label all T cells, T-helper cells and T-cytotoxic/suppressor cells, respectively. The resultant subsets were cultured in vitro with Con A. The supernatants were tested for the ability to induce MMC growth and differentiation in liquid cultures of normal rat bone marrow. The phenotype of the T cells producing this factor was established as being OX19+, W3/25+ and OX8-.
我们之前已经表明，来自感染巴西日圆线虫大鼠的淋巴细胞，在用抗原或伴刀豆球蛋白A（Con A）刺激时会释放出与小鼠白细胞介素-3相当的因子。将这些因子添加到大鼠骨髓培养物中，具有黏膜肥大细胞（MMC）形态和生化特性的肥大细胞会增殖并成熟。在此，我们利用这个系统，结合针对大鼠T细胞的单克隆抗体和荧光激活细胞分选仪（FACS），来分离负责产生这种MMC生长因子的T细胞亚群。来自感染巴西日圆线虫大鼠的淋巴细胞在FACS上被分离成带有和不带有由OX19、W3/25和OX8单克隆抗体所定义抗原的群体；这些抗体分别标记所有T细胞、T辅助细胞和T细胞毒性/抑制细胞。将所得亚群在体外与Con A一起培养。检测上清液在正常大鼠骨髓液体培养物中诱导MMC生长和分化的能力。产生这种因子的T细胞的表型被确定为OX19 +、W3/25 +和OX8 -。
