Department of Clinical Nutrition and Gastroenterological Diagnostics, Medical University of Lodz, 90-647 Lodz, Poland.
Department of Pharmaceutical Microbiology and Biochemistry, Medical University of Lodz, 92-215 Lodz, Poland.
Nutrients. 2024 Sep 4;16(17):2981. doi: 10.3390/nu16172981.
The causes of functional constipation (FC) in adults are unclear, but changes in the gut microbiome may play an important role. The present study aimed to assess the relationship between urinary metabolites of dopamine and serotonin and some dysbiosis indicators in patients with FC. The study included 40 healthy women and 40 women with FC aged 21-46 years.
Urinary levels of homovanillic acid (HVA), 5-hydoxyindoleacetic acid (5-HIAA), p-hydroxyphenylacetic acid (PhAc), and 3-indoxyl sulfate, as final metabolites of dopamine, serotonin, and indole pathway, respectively, were determined using the LC-Ms/Ms method. However, hydrogen-methane and ammonia breath tests were performed. The GA-map Dysbiosis Test was used to identify and characterize the dysbiosis index (DI).
In patients with FC, the DI was significantly higher than in the control group: 4.05 ± 0.53 vs. 1.52 ± 0.81 points ( < 0.001), but the number of many types of bacteria varied among individuals. The levels of HVA were higher, while 5-HIAA levels were lower in patients. Moreover, the HVA/5-HIAA ratio had a positive correlation with DI as well as with the severity of symptoms.
In patients with functional constipation, the balance in dopamine and serotonin secretion is disturbed, which is associated with changes in the gut microbiome.
成人功能性便秘（FC）的病因尚不清楚，但肠道微生物组的变化可能起着重要作用。本研究旨在评估 FC 患者的多巴胺和 5-羟色胺尿代谢物与一些肠道失调指标之间的关系。该研究纳入了 40 名健康女性和 40 名 21-46 岁 FC 女性。
采用 LC-MS/MS 法测定同型香草酸（HVA）、5-羟吲哚乙酸（5-HIAA）、对羟苯乙酸（PhAc）和 3-吲哚硫酸作为多巴胺、5-羟色胺和吲哚途径的终末代谢物的尿水平。然而，氢甲烷和氨呼气试验也进行了。GA-map 肠道失调测试用于识别和描述肠道失调指数（DI）。
在 FC 患者中，DI 明显高于对照组：4.05 ± 0.53 与 1.52 ± 0.81 分（<0.001），但个体之间的许多细菌类型的数量不同。HVA 水平较高，而 5-HIAA 水平较低。此外，HVA/5-HIAA 比值与 DI 以及症状严重程度呈正相关。
在功能性便秘患者中，多巴胺和 5-羟色胺分泌的平衡被打乱，这与肠道微生物组的变化有关。
