Carlin J M, Jensen J B, Geary T G
Am J Trop Med Hyg. 1985 Jul;34(4):668-74. doi: 10.4269/ajtmh.1985.34.668.
A variety of known or suspected inducers of crisis form parasites in cultivated Plasmodium falciparum were examined. Sera from Sudanese residents of malaria-endemic areas, sera from American tuberculosis patients, and rabbit sera containing tumor necrosis factor were assayed in vitro for cytotoxic activities against P. falciparum and mouse L-M cell cultures. Inhibition was determined by measurement of incorporation of radiolabeled nucleic acid precursors. When compared to normal serum, parasites grown in the presence of a 1:4 dilution of rabbit sera containing tumor necrosis factor, TB patient sera, or Sudanese sera were metabolically inhibited 73%, 75%, and 95%, respectively. However, only the rabbit sera containing tumor necrosis factor were cytotoxic to L-M cells, inhibiting radiolabel incorporation by 80% at a 1:1,000 serum dilution. These findings suggest that tumor necrosis factor is apparently not responsible for the induction of parasite crisis forms by the inhibitory human sera tested. In addition, human gamma-interferon had no effect on parasite growth.
对多种已知或疑似会在培养的恶性疟原虫中形成危机形式寄生虫的诱导物进行了检测。对来自疟疾流行地区的苏丹居民血清、美国结核病患者血清以及含有肿瘤坏死因子的兔血清进行了体外检测，以测定其对恶性疟原虫和小鼠L-M细胞培养物的细胞毒性活性。通过测量放射性标记核酸前体的掺入来确定抑制作用。与正常血清相比，在含有肿瘤坏死因子的兔血清、结核病患者血清或苏丹血清1:4稀释液存在下生长的寄生虫，其代谢分别被抑制了73%、75%和95%。然而，只有含有肿瘤坏死因子的兔血清对L-M细胞具有细胞毒性，在血清稀释1:1000时，放射性标记掺入被抑制了80%。这些发现表明，肿瘤坏死因子显然不是所检测的抑制性人血清诱导寄生虫危机形式的原因。此外，人γ干扰素对寄生虫生长没有影响。
