Wirtu Shuma Fayera, Ramaswamy Krishnaraj, Maitra Rahul, Chopra Sidharth, Mishra Ashutosh Kumar, Jule Leta Tesfaye
Department of Chemistry, College of Natural and Computational Science, Dambi Dollo University, Dambi Dollo, Ethiopia.
Department of Chemistry, Indian Institute of Technology, Hyderabad, 502285, India.
Sci Rep. 2024 Sep 16;14(1):21573. doi: 10.1038/s41598-024-71012-2.
Herbal medicines are important for ensuring sustainable development goals (SDGs) in healthcare, particularly in developing countries with high rates of antimicrobial resistance (AMR) and little access to medical facilities. Thymus vulgaris is a widely used herbal medicinal plant known for its secondary metabolites and antimicrobial properties. The present study involved a comprehensive examination of the isolation, characterization, and antibacterial activity of Thymus vulgaris obtained from Ethiopia. The aerial part of the plant Thymus vulgaris was successively extracted with hexane, chloroform, and methanol based on differences in polarity. Phytochemical screening tests conducted against hexane, chloroform and MeOH crude extracts indicated the presence of some secondary metabolites. Based on the thin-layer chromatography tests, the chloroform extract was subjected to column chromatography, yielding Tv-2 compounds, namely 5-isopropyl-2-methylphenol. The structures of the compounds were elucidated via spectroscopic methods (UV-Vis, FT-IR and NMR). We investigated the antibacterial properties of hexane crude extract, chloroform crude extract, MeOH crude extract, and isolated fractions derived from T. vulgaris against various bacterial strains. This study contributes to a better understanding of the bioactive components present in Thymus vulgaris crude extracts and their potential role in tackling microbial infections.
草药对于确保医疗保健领域的可持续发展目标（SDGs）至关重要，尤其是在抗菌药物耐药性（AMR）发生率高且医疗设施获取机会少的发展中国家。百里香是一种广泛使用的草药植物，以其次生代谢产物和抗菌特性而闻名。本研究对从埃塞俄比亚获得的百里香的分离、表征和抗菌活性进行了全面研究。基于极性差异，依次用己烷、氯仿和甲醇对百里香植物的地上部分进行提取。对己烷、氯仿和甲醇粗提物进行的植物化学筛选试验表明存在一些次生代谢产物。基于薄层色谱试验，氯仿提取物进行柱色谱分离，得到Tv-2化合物，即5-异丙基-2-甲基苯酚。通过光谱方法（紫外可见光谱、傅里叶变换红外光谱和核磁共振）阐明了化合物的结构。我们研究了百里香的己烷粗提物、氯仿粗提物、甲醇粗提物以及分离组分对各种细菌菌株的抗菌性能。本研究有助于更好地了解百里香粗提物中存在的生物活性成分及其在应对微生物感染中的潜在作用。
