果蝇中的化学诱变测试。III. 为国家毒理学计划测试的48种编码化合物的结果。

Chemical mutagenesis testing in Drosophila. III. Results of 48 coded compounds tested for the National Toxicology Program.

作者信息

Valencia R, Mason J M, Woodruff R C, Zimmering S

出版信息

Environ Mutagen. 1985;7(3):325-48. doi: 10.1002/em.2860070309.

Abstract

Forty-eight chemicals were tested for mutagenicity in Drosophila melanogaster. Each compound was tested first for the induction of sex-linked recessive lethals (SLRLs) by feeding the chemical in a solution of 5% aqueous sucrose. If it was not mutagenic by this route, the chemical was tested by injection in a solution of 0.7% aqueous NaCl. If found inactive, the compound was not tested further. If it was mutagenic, the compound was tested for the induction of reciprocal translocations (RTs) using the exposure protocol and germ cell stage that yielded positive results in the SLRL assay. Eight chemicals (diglycidylresorcinol ether, AF-2, alloxan monohydrate, 1-aziridine ethanol, p,p'-DDE, dichloroacetonitrile, 1,3-dichloropropene, and 3,3'-dimethylbenzidine dihydrochloride) were found to induce SLRLs. Only diglycidylresorcinol ether also induced RTs.

摘要

对48种化学物质进行了黑腹果蝇致突变性测试。每种化合物首先通过在5%蔗糖水溶液中喂食来测试其对性连锁隐性致死（SLRLs）的诱导作用。如果通过该途径没有致突变性，则将该化学物质在0.7%氯化钠水溶液中注射进行测试。如果发现无活性，则该化合物不再进一步测试。如果具有致突变性，则使用在SLRL测定中产生阳性结果的暴露方案和生殖细胞阶段对该化合物进行相互易位（RTs）诱导测试。发现8种化学物质（二缩水甘油间苯二酚醚、AF-2、一水合四氧嘧啶、1-氮丙啶乙醇、p,p'-滴滴伊、二氯乙腈、1,3-二氯丙烯和二盐酸3,3'-二甲基联苯胺）可诱导SLRLs。只有二缩水甘油间苯二酚醚也能诱导RTs。