Yoon Jaewoo, Yasumoto-Hirose Mina, Kasai Hiroaki
College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 42601, Republic of Korea.
Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate, 026-0001, Japan.
Folia Microbiol (Praha). 2025 Apr;70(2):455-462. doi: 10.1007/s12223-024-01200-0. Epub 2024 Sep 27.
A novel Gram-stain-negative, strictly aerobic, rod-shaped, light-yellow-pigmented, and chemo-organoheterotrophic bacterium, designated DF-77, was isolated from dense mats of filamentous algae collected in March 2004 at Okinawa in Japan. The microorganism grew at 0-2.0% NaCl concentrations (w/v), pH 6.0-9.0, and 20-30 °C. The 16S rRNA gene sequence-based phylogenetic tree demonstrated that the strain DF-77 is a novel member of the family Flavobacteriaceae and was greatly related to Flagellimonas nanhaiensis SM1704 with sequence similarity of 95.5%. The main fatty acids were iso-C G, iso-C, and iso-C 3-OH, and the only isoprenoid quinone was menaquinone-6. The dominant polar lipids were phosphatidylethanolamine, two unidentified aminolipids, an unidentified phosphoaminolipid, and four unidentified lipids. The genome size of strain DF-77 was 3.60 Mbp with a DNA G + C content of 47.5%. The average nucleotide identity (ANI) value between the genomes of strain DF-77 and its closely related species was 69.8-70.7%. The digital DNA - DNA hybridization (dDDH) value of strain DF-77 with the strain of F. nanhaiensis SM1704 was 16.8%. The genome of the strain DF-77 revealed that it encoded several genes involved in bio-macromolecule degradation, indicating a high potential for producing industrially useful enzymes. Consequently, the strain is described as a new species in the genus Flagellimonas, for which the name Flagellimonas algarum sp. nov., is proposed with the type strain DF-77 (= KCTC 72791 = NBRC 114251).
从2004年3月在日本冲绳采集的丝状藻密集垫中分离出一株新型革兰氏阴性、严格需氧、杆状、浅黄色色素化的化能有机异养细菌，命名为DF-77。该微生物在0-2.0%（w/v）的NaCl浓度、pH 6.0-9.0和20-30°C条件下生长。基于16S rRNA基因序列的系统发育树表明，菌株DF-77是黄杆菌科的一个新成员，与南海鞭毛单胞菌SM1704密切相关，序列相似性为95.5%。主要脂肪酸为异-C₁₅:₀、异-C₁₅:₁G和异-C₁₇:₀ 3-OH，唯一的类异戊二烯醌为甲基萘醌-6。主要极性脂为磷脂酰乙醇胺、两种未鉴定的氨基脂、一种未鉴定的磷酸氨基脂和四种未鉴定的脂。菌株DF-77的基因组大小为3.60 Mbp，DNA G+C含量为47.5%。菌株DF-77与其密切相关物种的基因组之间的平均核苷酸同一性（ANI）值为69.8-70.7%。菌株DF-77与南海鞭毛单胞菌SM1704菌株的数字DNA-DNA杂交（dDDH）值为16.8%。菌株DF-77的基因组显示其编码了几个参与生物大分子降解的基因，表明其具有产生工业上有用酶的高潜力。因此，该菌株被描述为鞭毛单胞菌属的一个新物种，提议将其命名为南海鞭毛单胞菌（Flagellimonas algarum sp. nov.），模式菌株为DF-77（=KCTC 72791=NBRC 114251）。
