Schieven B C, Hussain Z, Lannigan R
J Clin Microbiol. 1985 Oct;22(4):495-6. doi: 10.1128/jcm.22.4.495-496.1985.
This study compared the American MicroScan frozen microdilution tray and dehydrated microdilution tray. A total of 153 gram-negative and 106 gram-positive isolates were tested concurrently on both tray types and read visually. Very major and major antimicrobial susceptibility discrepancies amounted to 1.0% of all results, and minor discrepancies amounted to 0.4% of all results. The biochemical discrepancy rate for gram-negative organisms was 1.3%, resulting in no identification errors either at the genus or the species level. Gram-positive identification was not evaluated, as the formulation of biochemicals was changed after this evaluation was completed. Overall, the results with the dry tray were acceptable, providing a suitable alternative to the frozen tray.
本研究比较了美国MicroScan冷冻微量稀释板和脱水微量稀释板。总共153株革兰氏阴性菌和106株革兰氏阳性菌分离株同时在两种类型的平板上进行测试,并通过肉眼读取结果。极主要和主要的抗菌药物敏感性差异占所有结果的1.0%,次要差异占所有结果的0.4%。革兰氏阴性菌的生化差异率为1.3%,在属或种水平上均未导致鉴定错误。由于在完成此评估后改变了生化试剂的配方,因此未对革兰氏阳性菌鉴定进行评估。总体而言,干燥平板的结果是可接受的,为冷冻平板提供了合适的替代方案。