AutoSCAN-3和Sceptor系统用于肠杆菌科细菌鉴定的评估。
Evaluation of the AutoSCAN-3 and Sceptor systems for Enterobacteriaceae identification.
作者信息
Woolfrey B F, Lally R T, Quall C O
出版信息
J Clin Microbiol. 1983 May;17(5):807-13. doi: 10.1128/jcm.17.5.807-813.1983.
To evaluate the accuracy and cost effectiveness of the AutoSCAN-3 (Micro-Scan Systems of America, Sacramento, Calif.) and Sceptor (BBL Microbiology Systems, Cockeysville, Md.) systems for identification of members of the Enterobacteriaceae, we performed parallel tests on 678 stock cultures of well-characterized clinical isolates of Enterobacteriaceae. Automated results by AutoSCAN-3 correctly identified 95.1% at the genus level and 94.9% at the species level. However, 15 of 42 Shigella isolates were misidentified as members of other genera. In contrast to the automated results, visual interpretation of panels produced 97.9% agreement at the genus level, missing only three Shigella isolates. Sceptor correctly identified 96.8% at the genus level and 93.4% at the species level. Of 42 Shigella isolates, 3 were missed and were designated as Salmonella spp. Although all Salmonella spp. were correctly identified, six other isolates were misidentified as Salmonella spp. Test costs were found to be comparable for each system, with the cost per test increasing markedly with fewer than 10 to 15 tests performed per day.
为评估AutoSCAN - 3（美国加利福尼亚州萨克拉门托市的Micro - Scan Systems公司）和Sceptor（马里兰州科基斯维尔市的BBL微生物系统公司）系统对肠杆菌科细菌成员鉴定的准确性和成本效益，我们对678株特征明确的临床分离肠杆菌科菌株的储备培养物进行了平行测试。AutoSCAN - 3的自动化结果在属水平上正确鉴定率为95.1%，在种水平上为94.9%。然而，42株志贺氏菌分离株中有15株被错误鉴定为其他属的成员。与自动化结果相比，肉眼判读鉴定板在属水平上的符合率为97.9%，仅漏检3株志贺氏菌分离株。Sceptor在属水平上正确鉴定率为96.8%，在种水平上为93.4%。42株志贺氏菌分离株中，有3株漏检并被判定为沙门氏菌属。虽然所有沙门氏菌属均被正确鉴定，但另外6株分离株被错误鉴定为沙门氏菌属。发现每个系统的测试成本相当，每天进行的测试少于10至15次时，每次测试的成本会显著增加。
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