Nagar Vandan, Godambe Lipika Pansare, Newase Sandeep K, Tyagi Anuj
Food Technology Division, Bhabha Atomic Research Center, Mumbai, India.
Homi Bhabha National Institute, Mumbai, India.
Phage (New Rochelle). 2024 Sep 16;5(3):162-172. doi: 10.1089/phage.2024.0014. eCollection 2024 Sep.
The emergence of antibiotic-resistant strains presents a global health and aquaculture challenge. Bacteriophages offer promise as an alternative to antibiotics for treating drug-resistant infections.
Two new phages, P2 and vB_AhydM-H1, targeting pathogenic were isolated from sewage water. Their morphology, growth characteristics, lytic activity, stability, and genomes were analyzed.
Phage P2, a member of genus , and vB_AhydM-H1, a novel member of genus , exhibited narrow host ranges, extended latent periods, and typical burst sizes. Both phages remained stable at 40°C for 1 h and within a pH range of 4 to 10 for 3 h. The genomes of P2 and vB_AhydM-H1 spanned 42,660 bp with 49 open reading frames (ORFs) and 52,614 bp with 72 ORFs, respectively. Proteomic (ViPTree) and phylogenetic (VICTOR) analyses confirmed that both phages aligned with their respective families. DeepTMHMM predictions suggested that P2 and vB_AhydM-H1 encode three and four ORFs with transmembrane domains, respectively.
Safe for environmental and clinical use because of their lytic nature, and lack of virulence and resistance genes, these newly isolated phages expand the arsenal against antibiotic-resistant infections.
抗生素耐药菌株的出现给全球卫生和水产养殖带来了挑战。噬菌体有望成为治疗耐药性感染的抗生素替代品。
从污水中分离出两种靶向病原菌的新型噬菌体P2和vB_AhydM-H1。分析了它们的形态、生长特性、裂解活性、稳定性和基因组。
噬菌体P2属于 属，vB_AhydM-H1是 属的一个新成员，宿主范围狭窄，潜伏期延长，裂解量典型。两种噬菌体在40°C下1小时内保持稳定，在pH值4至10的范围内3小时内保持稳定。P2和vB_AhydM-H1的基因组分别跨越42,660 bp，有49个开放阅读框（ORF）和52,614 bp，有72个ORF。蛋白质组学（ViPTree）和系统发育（VICTOR）分析证实，两种噬菌体与其各自的家族一致。DeepTMHMM预测表明，P2和vB_AhydM-H1分别编码三个和四个带有跨膜结构域的ORF。
这些新分离的噬菌体因其裂解性质、缺乏毒力和抗性基因而对环境和临床使用安全，它们扩大了对抗生素耐药性感染的武器库。
