Bioinformatics Analysis of the Cyclophilin Gene and Its Anti- Activity.

In this paper, cyclophilin (PgCyP) was successfully obtained through a genetic engineering technique. A bioinformatics method was used to analyze the physicochemical properties and structure of . The results showed that belongs to the gene family. The protein encoded by the gene contains the active site of PPIase (R62, F67, and H133) and a binding site for A (W128). The relative molecular weight of PgCyP is 187.11 bp; its theoretical isoelectric point is 7.67, and it encodes 174 amino acids. The promoter region of mainly contains the low-temperature environmental stress response (LTR) element, abscisic acid-responsive cis-acting element (ABRE), and light-responsive cis-acting element (G-Box). includes a total of nine phosphorylation sites, comprising four serine phosphorylation sites, three threonine phosphorylation sites, and two tyrosine phosphorylation sites. was recombined and expressed in vitro, and its recombinant expression was investigated. Furthermore, it was found that the recombinant protein could effectively inhibit the germination of spores and the normal growth of mycelia in vitro. Further experiments on the roots of susceptible showed that the protein could improve the resistance of arabidopsis to . The findings of this study provide a basis for the use of the protein as a new type of green biopesticide.