Ben-Rafael Z, Kopf G S, Blasco L, Tureck R W, Mastroianni L
Fertil Steril. 1986 Jan;45(1):58-62. doi: 10.1016/s0015-0282(16)49097-7.
The results of reinsemination of 150 morphologically mature oocytes that failed to fertilize within 15 to 20 hours after initial insemination were evaluated. Only 41 oocytes (27.3%) were fertilized after reinsemination, which is significantly lower than the percentage obtained after initial insemination (74%). However, the cleavage rate was not impaired significantly. Polyspermic fertilization was common (17 oocytes, 29.3%). A total of 64 oocytes were transferred. In 18 patients, all of the oocytes that were transferred had been reinseminated; none of these patients conceived. However, of 28 patients who received embryos arising from both initial insemination and reinsemination protocols, 7 conceived (25%). We suggest that the percentage of fertilization is impaired after 22 hours of incubation in vitro and that those oocytes that have fertilized after reinsemination might partly reflect a misjudgment of initial oocyte maturity. Nevertheless, reinsemination is a relatively simple procedure, with no known attributed risk. Therefore, we recommend reinsemination to increase the overall fertilization percentage. Additional studies are needed to establish better methods for assessment of oocyte maturity to evaluate properly the benefit of such reinsemination procedures.
对150枚形态学成熟的卵母细胞进行再授精，这些卵母细胞在初次授精后15至20小时内未受精，对其结果进行了评估。再授精后仅有41枚卵母细胞（27.3%）受精，这显著低于初次授精后的受精率（74%）。然而，卵裂率并未受到显著影响。多精受精很常见（17枚卵母细胞，29.3%）。总共移植了64枚卵母细胞。在18例患者中，所有移植的卵母细胞均经过再授精；这些患者均未受孕。然而，在28例接受初次授精和再授精方案产生的胚胎的患者中，7例受孕（25%）。我们认为，体外培养22小时后受精率会受到影响，而再授精后受精的那些卵母细胞可能部分反映了对初始卵母细胞成熟度的误判。尽管如此，再授精是一个相对简单的程序，没有已知的相关风险。因此，我们建议进行再授精以提高总体受精率。需要进一步研究以建立更好的卵母细胞成熟度评估方法，从而正确评估此类再授精程序的益处。
