Boldt J, Howe A M, Butler W J, McDonough P G, Padilla S L
Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta 30912.
Fertil Steril. 1987 Oct;48(4):617-23. doi: 10.1016/s0015-0282(16)59474-6.
The value of oocyte reinsemination in human in vitro fertilization (IVF) was evaluated in this study. Two groups of 25 cycles each were studied. In group 1 cycles, insemination of mature/questionably mature oocytes (judged by cumulus and corona cell appearance) at 6.5 hours postcollection yielded a fertilization rate of 42.3%. This value was significantly lower than that obtained with reinsemination (70.8%). In group 2, questionably mature oocytes were treated as immature and inseminated 24 to 30 hours postcollection. The fertilization rate of mature eggs in group 2 was 68.2%, with a reinsemination success rate of 40%. Triploidy rates and cleavage rates of mature eggs, fertilized either at initial insemination or reinsemination and of fertilized immature eggs, were similar within group 1 and group 2 cycles. In group 1, two pregnancies resulted from transfer of embryos arising solely from successful reinsemination, with one infant delivered. These results indicate that reinsemination offers a useful adjunct for IVF in cases where fertilization failure occurs.
本研究评估了人体外受精（IVF）中卵母细胞再授精的价值。对两组各25个周期进行了研究。在第1组周期中，对采集后6.5小时的成熟/可疑成熟卵母细胞（根据卵丘和放射冠细胞外观判断）进行授精，受精率为42.3%。该值显著低于再授精时获得的值（70.8%）。在第2组中，将可疑成熟卵母细胞视为未成熟卵母细胞，并在采集后24至30小时进行授精。第2组中成熟卵子的受精率为68.2%，再授精成功率为40%。第1组和第2组周期内，初次授精或再授精的成熟卵子以及受精的未成熟卵子的三倍体率和分裂率相似。在第1组中，仅因成功再授精产生的胚胎移植导致了两次妊娠，产下一名婴儿。这些结果表明，在发生受精失败的情况下，再授精为体外受精提供了一种有用的辅助手段。
