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鸡H1组蛋白基因保守5'元件的诱变与转录

Mutagenesis of conserved 5' elements and transcription of a chicken H1 histone gene.

作者信息

Younghusband H B, Sturm R, Wells J R

出版信息

Nucleic Acids Res. 1986 Jan 24;14(2):635-44. doi: 10.1093/nar/14.2.635.

DOI:10.1093/nar/14.2.635
PMID:3945554
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC339454/
Abstract

In addition to readily identifiable TATA and CAAT boxes, chicken H1 histone genes contain a highly conserved G-rich region at about minus 80 and an A-rich H1 histone gene-specific motif some 120 bases upstream from the H1 mRNA cap site. The level of transcription from wild-type, 'G-box' and 'A-box' H1 deletion mutant templates was tested in Xenopus oocytes and in HeLa cells. Removal of the H1 gene-specific motif had no effect on H1 gene transcription in either assay system, whereas deletion of the G-rich sequence decreased H1 mRNA levels by about ten-fold in oocytes and in HeLa cells. At least in heterologous systems, the H1-gene specific A-rich region does not appear to influence the level of H1 gene transcription.

摘要

除了易于识别的TATA盒和CAAT盒外，鸡H1组蛋白基因在约-80处含有一个高度保守的富含G的区域，以及一个富含A的H1组蛋白基因特异性基序，位于H1 mRNA帽位点上游约120个碱基处。在非洲爪蟾卵母细胞和HeLa细胞中测试了野生型、“G盒”和“A盒”H1缺失突变体模板的转录水平。在任一检测系统中，去除H1基因特异性基序对H1基因转录均无影响，而富含G的序列缺失使卵母细胞和HeLa细胞中的H1 mRNA水平降低约10倍。至少在异源系统中，H1基因特异性富含A的区域似乎不影响H1基因的转录水平。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c66f/339454/e600a45c2197/nar00271-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c66f/339454/cb5b72c87156/nar00271-0027-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c66f/339454/75961f4574d5/nar00271-0028-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c66f/339454/ec8be41a6dd5/nar00271-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c66f/339454/e600a45c2197/nar00271-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c66f/339454/cb5b72c87156/nar00271-0027-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c66f/339454/75961f4574d5/nar00271-0028-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c66f/339454/ec8be41a6dd5/nar00271-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c66f/339454/e600a45c2197/nar00271-0031-a.jpg

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