Jung Katja, Kerzel Peter, Hara Anderson T, Luka Benedikt, Schlueter Nadine, Ganss Carolina
Department of Conservative and Preventive Dentistry, Dental Clinic, Justus Liebig University, Giessen, Germany.
Section Cariology, Department of Operative Dentistry, Endodontics and Paediatric Dentistry, Philipps University, Marburg, Germany.
Caries Res. 2025;59(2):139-150. doi: 10.1159/000542178. Epub 2024 Oct 25.
Hydroxyapatite (HAP) is promoted as biomimetic material in dentistry. The aim of the study was to investigate whether HAP-containing formulations can reduce erosive/abrasive tissue loss and to analyse components in these formulations.
Two HAP toothpastes with and two without fluoride and a HAP mouthrinse were investigated, controls were active agent-free toothpaste, SnF2 toothpaste and F/Sn mouthrinse. For 10 days, human enamel samples were eroded for 2 min, 6×/day in 0.5% citric acid and immersed for 2 min, 2×/day in toothpaste slurries or mouthrinse. Half were additionally brushed for 15 s, 2×/day. The particulate fraction was extracted and examined morphologically and with element analyses. Other parameters were REA, RDA, fluoride and calcium content.
The F/Sn mouthrinse almost completely prevented tissue loss; none of the HAP formulations reduced tissue loss compared to the negative control, two increased it instead. Brushing increased tissue loss in all groups except the F/Sn mouthrinse. All toothpastes contained amorphous particles of different sizes. Elemental analysis identified Si and O, and additionally, Ca and P were present in small amounts on the particles of the HAP toothpastes and one HAP+F toothpaste. In the liquid phase, elevated calcium levels were found in one HAP toothpaste and in both HAP+F toothpastes; in the formulation with the highest value, the fluoride concentration was low. REA and RDA values were not associated with tissue loss.
Whether alone or in combination with fluoride, HAP formulations had either no or a detrimental effect on erosive tissue loss and could not reduce abrasion. In the context of erosive tooth wear, HAP seems to be neither an alternative to fluoride nor a suitable supplement to it.
羟基磷灰石（HAP）在牙科领域被推崇为仿生材料。本研究的目的是调查含HAP的制剂是否能减少侵蚀性/磨蚀性组织损失，并分析这些制剂中的成分。
研究了两种含氟和两种不含氟的HAP牙膏以及一种HAP漱口水，对照分别为不含活性剂的牙膏、SnF2牙膏和F/Sn漱口水。人牙釉质样本在0.5%柠檬酸中每天侵蚀6次，每次2分钟，持续10天，并每天2次浸泡在牙膏糊剂或漱口水2分钟。其中一半样本每天额外刷牙2次，每次15秒。提取颗粒部分并进行形态学检查和元素分析。其他参数包括相对侵蚀活性（REA）、相对磨损率（RDA）、氟化物和钙含量。
F/Sn漱口水几乎完全防止了组织损失；与阴性对照相比，没有一种HAP制剂减少组织损失，反而有两种增加了组织损失。除F/Sn漱口水组外，刷牙在所有组中均增加了组织损失。所有牙膏都含有不同大小的无定形颗粒。元素分析鉴定出硅和氧，此外，在HAP牙膏和一种含氟HAP牙膏的颗粒上还存在少量的钙和磷。在液相中，一种HAP牙膏和两种含氟HAP牙膏中钙水平升高；在钙含量最高的制剂中，氟化物浓度较低。REA和RDA值与组织损失无关。
无论是单独使用还是与氟化物联合使用，HAP制剂对侵蚀性组织损失要么没有影响，要么有不利影响，且不能减少磨损。在侵蚀性牙齿磨损的情况下，HAP似乎既不是氟化物的替代品，也不是合适的补充剂。
