Donayre-Salvatierra Gina Alessandra, Dulanto-Vargas Julissa Amparo, Olaechea Raul M, Reátegui Oscar, Carranza-Samanez Kilder Maynor
School of Dentistry, Universidad Científica del Sur, Lima, Peru.
Research Group in Dental Sciences, School of Dentistry, Universidad Científica del Sur, Lima, Peru.
Int J Dent. 2024 Oct 18;2024:3509832. doi: 10.1155/2024/3509832. eCollection 2024.
Oral hygiene education for patients is fundamental in preventive-promotional dentistry. The disinfection of toothbrushes (TBs) must be integrated into this context due to their proximity to contaminant sources that make them vulnerable to cross infection in homes. The objective of this study was to compare the efficacy of domestic techniques for disinfection of TBs. We performed an in vitro study of 76 TBs contaminated with () ATCC 29212 subjected to different disinfection protocols: G1. Distilled water (DW; negative control; = 8), G2. Dimethylsulfoxide (DMSO)10% (negative control; = 8), G3. Chlorhexidine (CHX) 0.12% (positive control; = 15), G4. 100% white vinegar (WV; = 15), G5. Microwave (MW) at 700 W ( = 15), and G6. 200 mg/mL of certified alcoholic extract of purple garlic (GARLIC) from Arequipa (; = 15). Bacterial count was assessed by colony-forming units (CFU/mL) categorized as contamination: low (<30), medium (30-300), and high (>300). The Kruskal-Wallis test with post hoc pairs was used at a significance level of < 0.05. Efficacy against showed highly significant differences between groups ( < 0.001) with lower median CFU/mL in G3 and G4 (Me = 0 [IQR (interquartile range) = 0]: low) and G5 (Me = 6000 [IQR = 45,000]: low/medium) versus negative controls (Me = 378,500 and 5,020,000 [IQR = 4,605,000 and 6,760,000]: medium/high; ≤ 0.019). The counts of the G5 were not statistically different than G3, G4, and G6 ( > 0.06). The G6 (Me = 1,510,000 [IQR = 590,000]: medium) was inferior to G3 and G4 ( < 0.001), but similar to both negative control groups ( > 0.999). Disinfection of TBs with CHX, WV, and MWs produces a significant effective reduction in the count of .
对患者进行口腔卫生教育是预防 - 促进性牙科的基础。由于牙刷（TBs）靠近污染源，使其在家庭中易受交叉感染，因此牙刷消毒必须纳入这一范畴。本研究的目的是比较家庭牙刷消毒技术的效果。我们对76支被ATCC 29212污染的牙刷进行了体外研究，这些牙刷接受了不同的消毒方案：G1. 蒸馏水（DW；阴性对照；n = 8），G2. 10%二甲亚砜（DMSO；阴性对照；n = 8），G3. 0.12%氯己定（CHX；阳性对照；n = 15），G4. 100%白醋（WV；n = 15），G5. 700瓦微波（MW；n = 15），以及G6. 来自阿雷基帕的200mg/mL认证紫大蒜酒精提取物（大蒜；n = 15）。通过菌落形成单位（CFU/mL）评估细菌计数，并将其分类为污染程度：低（<30）、中（30 - 300）和高（>300）。采用Kruskal - Wallis检验及事后配对检验，显著性水平设定为p < 0.05。对金黄色葡萄球菌的消毒效果显示，各实验组之间存在高度显著差异（p < 0.001），G3和G4组（中位数 = 0 [四分位间距（IQR） = 0]：低污染）以及G5组（中位数 = 6000 [IQR = 45000]：低/中污染）的CFU/mL中位数低于阴性对照组（中位数 = 378500和5020000 [IQR = 4605000和6760000]：中/高污染；p ≤ 0.019）。G5组的计数与G3、G4和G6组相比无统计学差异（p > 0.06）。G6组（中位数 = 1510000 [IQR = 590000]：中污染）不如G3和G4组（p < 0.001），但与两个阴性对照组相似（p > 0.999）。用CHX、WV和MW对牙刷进行消毒可显著有效降低金黄色葡萄球菌的计数。
