Sano Kayoko, Soga Yuka, Ohta Kaori, Kitamura Yuki, Arimoto-Kobayashi Sakae
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, 700-8530, Japan.
School of Pharmaceutical Sciences, Okayama University, Okayama, 700-8530, Japan.
Genes Environ. 2024 Oct 28;46(1):22. doi: 10.1186/s41021-024-00317-5.
We previously investigated methods for separating mutagenic contaminants from aqueous solutions using cellulose-bearing covalently bound trisulfo-Cu-phthalocyanine (blue cotton and blue rayon). Mutagenic contaminants with three or more fused aromatic rings in their structures were adsorbed onto blue cotton and rayon. Since Cu-phthalocyanine is considered an unsuitable absorption ligand for byproducts of water chlorination, such as 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (Mutagen X or MX), we investigated the development of a new material for the elimination of MX from aqueous solvents.
We selected green cellulose powder bearing ferrous phthalocyanine (FePh), hereafter referred to as green cellulose or GP, as the candidate material. GP is composed of cationized cellulose (white cellulose, WP) and FePh tetracarboxylic acid. The mutagenicity of MX dissolved in buffer or dimethyl sulfoxide (DMSO) solution significantly decreased after treatment with GP. The effects of GP on the elimination of MX from the solvent were very close to being expired after 70 cycles of repeated adsorption of the same GP, and the capacity of GP for MX removal was estimated to be exhausted after 120 cycles of repeated adsorption based on the extrapolation of the obtained result; thus, the interacting ligands on GP may be saturated after complete MX adsorption. The mutagenicity of MX dissolved in aqueous buffer significantly decreased after treatment at pH7.4 but not at pH 4.0. Since MX is dissociated to be the anionic form at pH 6 or higher, the negative charge of MX in the buffer at pH 7.4 may interact with the positive charge of ferrous ions in GP to create a linkage between MX and GP. After GP adsorbed MX, mutagenicity was extracted with water or acetonitrile and recovered in the eluent. Thus, the reversible interaction between MX and FePh may have caused adsorption of MX onto GP.
GP could be used as a new eliminator and recovery agent for MX in chlorinated drinking water. Developing new materials for the removal and recovery of agents for the detection of mutagenic contaminant-related chlorination in water is beneficial for environmental health.
我们之前研究了使用负载纤维素的共价结合三磺基铜酞菁（蓝棉和蓝粘胶纤维）从水溶液中分离诱变污染物的方法。结构中含有三个或更多稠合芳环的诱变污染物会吸附在蓝棉和粘胶纤维上。由于酞菁铜被认为是水氯化副产物（如3 - 氯 - 4 -（二氯甲基）- 5 - 羟基 - 2（5H）- 呋喃酮（诱变剂X或MX））的不合适吸附配体，我们研究了一种从水性溶剂中去除MX的新材料的开发。
我们选择负载亚铁酞菁（FePh）的绿色纤维素粉末（以下简称绿色纤维素或GP）作为候选材料。GP由阳离子化纤维素（白色纤维素，WP）和FePh四羧酸组成。用GP处理后，溶解在缓冲液或二甲基亚砜（DMSO）溶液中的MX的诱变性显著降低。在相同的GP重复吸附70次后，GP从溶剂中去除MX的效果几乎消失，根据所得结果外推，在重复吸附120次后，GP去除MX的能力估计耗尽；因此，在MX完全吸附后，GP上的相互作用配体可能会饱和。溶解在水性缓冲液中的MX在pH7.4处理后诱变性显著降低，但在pH 4.0时未降低。由于MX在pH 6或更高时会解离成阴离子形式，pH 7.4缓冲液中MX的负电荷可能与GP中亚铁离子的正电荷相互作用，从而在MX和GP之间形成联系。GP吸附MX后，用水或乙腈提取诱变性并在洗脱液中回收。因此，MX与FePh之间的可逆相互作用可能导致MX吸附到GP上。
GP可作为氯化饮用水中MX的新型去除剂和回收剂。开发用于去除和回收水中诱变污染物相关氯化检测试剂的新材料对环境健康有益。
