Faase Ryan A, Hummel Madeleine H, Hasbrook AnneMarie V, Carpenter Andrew P, Baio Joe E
School of Chemical Biological and Environmental Engineering, Oregon State University, Corvallis, OR 97331, USA.
Beilstein J Nanotechnol. 2024 Nov 8;15:1376-1389. doi: 10.3762/bjnano.15.111. eCollection 2024.
One biomimetic approach to surface passivation involves a series of surface coatings based on the slick surfaces of carnivorous pitcher plants (Nepenthes), termed slippery liquid-infused porous surfaces (SLIPS). This study introduces a simplified method to produce SLIPS using a polydopamine (PDA) anchor layer, inspired by mussel adhesion. SLIPS layers were formed on cyclic olefin copolymer, silicon, and stainless steel substrates, by first growing a PDA film on each substrate. This was followed by a hydrophobic liquid anchor layer created by functionalizing the PDA film with a fluorinated thiol. Finally, perfluorodecalin was applied to the surface immediately prior to use. These biomimetic surface functionalization steps were confirmed by several complimentary surface analysis techniques. The wettability of each surface was probed with water contact angle measurements, while the chemical composition of the layer was determined by X-ray photoelectron spectroscopy. Finally, ordering of specific chemical groups within our PDA SLIPS layer was determined via sum frequency generation spectroscopy. The hemocompatibility of our new PDA-based SLIPS coating was then evaluated by tracking FXII activation, fibrin generation time, clot morphology, and platelet adhesion to the surface. This hemocompatibility work suggests that PDA SLIPS coatings slow or prevent clotting, but the observation of both FXII activation and the presence of adherent and activated platelets at the PDA SLIPS samples imply that this formulation of a SLIPS coating is not completely omniphobic.
一种用于表面钝化的仿生方法涉及一系列基于食肉猪笼草（猪笼草属）光滑表面的表面涂层，称为注入滑液的多孔表面（SLIPS）。本研究引入了一种受贻贝粘附启发、使用聚多巴胺（PDA）锚定层来制备SLIPS的简化方法。通过首先在环状烯烃共聚物、硅和不锈钢基底上生长PDA膜，在这些基底上形成SLIPS层。随后，通过用氟化硫醇对PDA膜进行功能化处理，创建一个疏水液体锚定层。最后，在使用前立即将全氟萘烷应用于表面。这些仿生表面功能化步骤通过几种互补的表面分析技术得到了证实。通过水接触角测量来探测每个表面的润湿性，同时通过X射线光电子能谱确定该层的化学成分。最后，通过和频振动光谱确定我们的PDA SLIPS层内特定化学基团的排列顺序。然后，通过跟踪FXII激活、纤维蛋白生成时间、血凝块形态以及血小板在表面的粘附情况，评估我们新的基于PDA的SLIPS涂层的血液相容性。这项血液相容性研究表明，PDA SLIPS涂层可减缓或防止凝血，但在PDA SLIPS样品上观察到的FXII激活以及粘附和活化血小板的存在意味着这种SLIPS涂层配方并非完全具有疏液性。
