Ojaghi Ali, Jourkesh Morteza, Ojaghi Morteza, Mirheidar Lamia, Neshati Abolfazl
Department of Physical Education and Sports Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran.
Department of Exercise Physiology, Faculty of Physical Education, Ilkhichi Branch, Islamic Azad University, Ilkhichi, Iran.
Arch Med Sci Atheroscler Dis. 2024 Sep 28;9:e177-e182. doi: 10.5114/amsad/188093. eCollection 2024.
Although reliable new evidence has identified several advantages of resistance training (ResEx) on cardiac performance, the role of this type of training in protecting the myocardium against ischemia-reperfusion (IR) injury is not clear. The aim of this study was to investigate the effect of resistance training on cardioprotection versus IR-induced injury.
60-day-old male Wistar rats ( = 24), weighing 220-240 g, were divided into four groups: Resistance Training (ResEx), Isoproterenol (ISO), Resistance Training + Isoproterenol (ResEx + ISO), and control groups ( = 6 for each). Trained rats performed exercise in a squat-training apparatus (8-12 repetitions/set, eight sets/day, and 5 days/week for 8 weeks). After the last training session, all the rats were sacrificed with ketamine xylazine injection. The heart of rats was removed from the body and washed quickly with cold PBS, immediately put in liquid nitrogen, and stored at -70°C. Superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) activity was measured.
Induction of ischemia decreased SOD and CAT activity but had no effect on the activity of GPX. Eight weeks of resistance training significantly increased activity of SOD and CAT compared to the ISO group.
The results of the present research demonstrated that ischemia induced by isoproterenol injection during 8 weeks of resistance training did not lead to a decrease in SOD activity and prevented the reduction of CAT activity.
尽管可靠的新证据已证实抗阻训练（ResEx）对心脏功能有若干益处，但这种训练方式在保护心肌免受缺血再灌注（IR）损伤方面的作用尚不清楚。本研究旨在探讨抗阻训练对心脏保护作用及抗IR诱导损伤的影响。
选取60日龄雄性Wistar大鼠（n = 24），体重220 - 240克，分为四组：抗阻训练组（ResEx）、异丙肾上腺素组（ISO）、抗阻训练 + 异丙肾上腺素组（ResEx + ISO）和对照组（每组n = 6）。训练大鼠在深蹲训练器械上进行运动（每组8 - 12次重复，每天8组，每周5天，共8周）。最后一次训练结束后，所有大鼠经氯胺酮 - 赛拉嗪注射处死。取出大鼠心脏，用冷PBS快速冲洗，立即放入液氮中，并储存在 - 70°C。测定超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPX）和过氧化氢酶（CAT）活性。
缺血诱导降低了SOD和CAT活性，但对GPX活性无影响。与ISO组相比，8周的抗阻训练显著提高了SOD和CAT活性。
本研究结果表明，在8周抗阻训练期间，异丙肾上腺素注射诱导的缺血并未导致SOD活性降低，并防止了CAT活性的降低。
