Simpson H B, Tobias M L, Kelley D B
J Comp Neurol. 1986 Feb 22;244(4):430-44. doi: 10.1002/cne.902440403.
The central projections of individual components of the IX-X nerve complex in the South African clawed frog, Xenopus laevis, were mapped by dye diffusion with Lucifer Yellow in an isolated brain preparation. The method reliably revealed fiber tracts, termination zones, and detailed cell morphology. In addition, motor neurons could be doubly labelled by retrograde transport of horseradish peroxidase from muscle targets followed by backfilling the appropriate nerves with Lucifer Yellow. The most anterior root associated with the nerve IX-X complex, root 1, is composed of lateral line afferents that terminate in the medial medulla. Root 2 contains sensory fibers that terminate in the nucleus tractus solitarii and axons of lateral line efferent neurons. Root 3 is composed of sensory and motor fibers, including a major somatosensory component that terminates in posterior medulla and anterior spinal cord, and axons from cranial nerve nucleus IX-X. The most posterior root of the IX-X nerve complex, root 4, contains axons of laryngeal motor neurons and of general visceral efferent neurons.
在南非爪蟾（非洲爪蟾）的离体脑标本中，通过荧光黄染料扩散法绘制了第IX - X神经复合体各个组成部分的中枢投射图。该方法可靠地揭示了纤维束、终末区以及详细的细胞形态。此外，运动神经元可以通过辣根过氧化物酶从肌肉靶点逆行运输，随后用荧光黄对相应神经进行回充来进行双重标记。与第IX - X神经复合体相关的最前根，即根1，由终止于延髓内侧的侧线传入纤维组成。根2包含终止于孤束核的感觉纤维以及侧线传出神经元的轴突。根3由感觉和运动纤维组成，包括一个主要的躯体感觉成分，其终止于延髓后部和脊髓前部，以及来自第IX - X脑神经核的轴突。第IX - X神经复合体的最后根，即根4，包含喉运动神经元和一般内脏传出神经元的轴突。
