Zhao Zhikun, Ma Xiaoxiao, Li Mingyan, Chen Guangyuan, Qi Libo, Song Shuang, Li Zhenhao, Yan Chunhong
Liaoning Key Laboratory of Food Nutrition and Health, Collaborative Innovation Center of Seafood Deep Processing, National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116034, China.
Zhejiang Key Laboratory of Biological Breeding and Exploitation of Edible and Medicinal Mushrooms, Zhejiang Shouxiangu Institute of Rare Medicine Plant, Wuyi, 321200, Zhejiang, China.
Plant Foods Hum Nutr. 2024 Dec 5;80(1):2. doi: 10.1007/s11130-024-01271-x.
The hepatoprotective effect of the alcoholic extracts of Ganoderma lucidum fermentation products (GFE) was investigated. C57BL/6 mice were pretreated with GFE for 7 days and then subjected to the chronic-binge ethanol feeding model. GFE pretreatment significantly reduced the ethanol-induced elevated serum levels of aspartate aminotransferase (AST) and alanine transaminase (ALT), hepatic steatosis, and increased triglyceride content. GFE pretreatment also altered hepatic alcohol metabolism, suppressed oxidative stress by decreasing the expression of Cyp2e1, and increasing the level of GSH. Lipidmoic analysis revealed that GFE pretreatment effectively increased ratio of phosphatidylcholines /phosphatidylethanolamine (PC/PE) in the liver. Furthermore, mice pretreated with GFE demonstrated decreased hepatic inflammation and plasma lipopolysaccharide (LPS) levels. Additionally, the mRNA expression of gut tight junction proteins such as ZO-1, Occludin and Claudin-1, along with antimicrobial peptide (e.g., Reg3β and Reg3γ) were up-regulated by GFE pretreatment. 16s rRNA sequencing revealed that GFE increased Bacteroidales, Parabacteroides, and Dubosiella, which were associated with hepatic steatosis, inflammation and intestinal barrier function parameters. These results demonstrate that GFE can prevent ethanol-induced liver injury and inflammation, gut leakiness and restore gut microbiota dysbiosis.
研究了灵芝发酵产物乙醇提取物（GFE）的保肝作用。将C57BL/6小鼠用GFE预处理7天，然后采用慢性暴饮乙醇喂养模型。GFE预处理显著降低了乙醇诱导的血清天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）水平升高、肝脂肪变性以及甘油三酯含量增加。GFE预处理还改变了肝脏酒精代谢，通过降低Cyp2e1的表达和增加谷胱甘肽（GSH）水平来抑制氧化应激。脂质组学分析显示，GFE预处理有效提高了肝脏中磷脂酰胆碱/磷脂酰乙醇胺（PC/PE）的比例。此外，用GFE预处理的小鼠肝脏炎症和血浆脂多糖（LPS）水平降低。另外，GFE预处理上调了肠道紧密连接蛋白如闭合蛋白1（ZO-1）、闭合蛋白（Occludin）和Claudin-1以及抗菌肽（如再生胰岛衍生蛋白3β（Reg3β）和再生胰岛衍生蛋白3γ（Reg3γ））的mRNA表达。16s rRNA测序显示，GFE增加了拟杆菌目、副拟杆菌属和杜波氏菌属，这些与肝脂肪变性、炎症和肠道屏障功能参数相关。这些结果表明，GFE可以预防乙醇诱导的肝损伤和炎症、肠道渗漏并恢复肠道微生物群失调。
