• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

脑血管特异性细胞外基质生物墨水促进血脑屏障特性。

Cerebrovascular-Specific Extracellular Matrix Bioink Promotes Blood-Brain Barrier Properties.

作者信息

Han Hohyeon, Lee Sooyeon, Gao Ge, Yi Hee-Gyeong, Paek Sun Ha, Jang Jinah

机构信息

Division of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang 37666, Republic of Korea.

Department of Convergence IT Engineering, POSTECH, Pohang 37666, Republic of Korea.

出版信息

Biomater Res. 2024 Dec 5;28:0115. doi: 10.34133/bmr.0115. eCollection 2024.

DOI:10.34133/bmr.0115
PMID:39641002
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11617618/
Abstract

Chronic neuroinflammation is a principal cause of neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. The blood-brain barrier predominantly comprises endothelial cells, and their intercellular communication with pericytes and other cell types regulates neuroinflammation. Here, we develop a tubular, perfusable model of human cerebrovascular tissues to study neurodegenerative diseases using cerebrovascular-specific extracellular matrix bioink, derived from a complementary blend of brain- and blood-vessel-derived extracellular matrices. The endothelial cells and pericytes in the bioprinted constructs spontaneously self-assemble into a dual-layered structure, closely mimicking the anatomy of the blood-brain barrier. Moreover, the mature cerebrovascular tissue shows physiological barrier functions and neuroinflammatory responses, indicating its potential for developing models of neuroinflammation-related pathologies. Collectively, our study demonstrates that furnishing a cerebrovascular-specific microenvironment can guide the cells to have native-like anatomical relevance and functional recapitulation in vitro.

摘要

慢性神经炎症是阿尔茨海默病和帕金森病等神经退行性疾病的主要病因。血脑屏障主要由内皮细胞组成，它们与周细胞和其他细胞类型的细胞间通讯调节神经炎症。在这里，我们使用源自脑和血管衍生细胞外基质的互补混合物的脑血管特异性细胞外基质生物墨水，开发了一种用于研究神经退行性疾病的人体脑血管组织的管状、可灌注模型。生物打印构建体中的内皮细胞和周细胞自发地自组装成双层结构，紧密模拟血脑屏障的解剖结构。此外，成熟的脑血管组织显示出生理屏障功能和神经炎症反应，表明其在开发神经炎症相关病理模型方面的潜力。总的来说，我们的研究表明，提供脑血管特异性微环境可以引导细胞在体外具有类似天然的解剖相关性和功能重现性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85e2/11617618/2e787f6a45b5/bmr.0115.fig.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85e2/11617618/e41e956fe33d/bmr.0115.fig.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85e2/11617618/b712a39925ef/bmr.0115.fig.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85e2/11617618/4808c4b5d3aa/bmr.0115.fig.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85e2/11617618/1b037ac532a2/bmr.0115.fig.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85e2/11617618/7ed3a00e57d8/bmr.0115.fig.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85e2/11617618/2e787f6a45b5/bmr.0115.fig.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85e2/11617618/e41e956fe33d/bmr.0115.fig.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85e2/11617618/b712a39925ef/bmr.0115.fig.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85e2/11617618/4808c4b5d3aa/bmr.0115.fig.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85e2/11617618/1b037ac532a2/bmr.0115.fig.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85e2/11617618/7ed3a00e57d8/bmr.0115.fig.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85e2/11617618/2e787f6a45b5/bmr.0115.fig.006.jpg

相似文献

1
Cerebrovascular-Specific Extracellular Matrix Bioink Promotes Blood-Brain Barrier Properties.脑血管特异性细胞外基质生物墨水促进血脑屏障特性。
Biomater Res. 2024 Dec 5;28:0115. doi: 10.34133/bmr.0115. eCollection 2024.
2
Bioink with cartilage-derived extracellular matrix microfibers enables spatial control of vascular capillary formation in bioprinted constructs.含有软骨来源细胞外基质微纤维的生物墨水能够在生物打印构建物中对血管毛细血管形成进行空间控制。
Biofabrication. 2022 Apr 20;14(3). doi: 10.1088/1758-5090/ac6282.
3
Direct 3D bioprinting of perfusable vascular constructs using a blend bioink.使用混合生物墨水对可灌注血管构建体进行直接3D生物打印。
Biomaterials. 2016 Nov;106:58-68. doi: 10.1016/j.biomaterials.2016.07.038. Epub 2016 Aug 2.
4
3D self-organized microvascular model of the human blood-brain barrier with endothelial cells, pericytes and astrocytes.具有内皮细胞、周细胞和星形胶质细胞的人血脑屏障 3D 自组织微血管模型。
Biomaterials. 2018 Oct;180:117-129. doi: 10.1016/j.biomaterials.2018.07.014. Epub 2018 Jul 12.
5
Pericyte Control of Gene Expression in the Blood-Brain Barrier Endothelium: Implications for Alzheimer's Disease.周细胞对血脑屏障内皮基因表达的调控：阿尔茨海默病的相关影响。
J Alzheimers Dis. 2024;99(s2):S281-S297. doi: 10.3233/JAD-230907.
6
3D bioprinting of multi-layered segments of a vessel-like structure with ECM and novel derived bioink.使用细胞外基质（ECM）和新型衍生生物墨水对血管样结构的多层节段进行3D生物打印。
Front Bioeng Biotechnol. 2022 Aug 19;10:918690. doi: 10.3389/fbioe.2022.918690. eCollection 2022.
7
[Extracellular matrix remodeling as a risk factor for the progression of cerebrovascular pathology].[细胞外基质重塑作为脑血管病变进展的危险因素]
Zh Nevrol Psikhiatr Im S S Korsakova. 2022;122(3):27-31. doi: 10.17116/jnevro202212203127.
8
A Photo-Crosslinkable Kidney ECM-Derived Bioink Accelerates Renal Tissue Formation.一种光交联性肾脏细胞外基质衍生生物墨水加速肾脏组织形成。
Adv Healthc Mater. 2019 Apr;8(7):e1800992. doi: 10.1002/adhm.201800992. Epub 2019 Feb 6.
9
An engineered neurovascular unit for modeling neuroinflammation.一种用于模拟神经炎症的工程化神经血管单元。
Biofabrication. 2021 May 5;13(3). doi: 10.1088/1758-5090/abf741.
10
Perfusable cell-laden matrices to guide patterning of vascularization .可灌注的载细胞基质用于引导血管化的模式形成。
Biomater Sci. 2023 Jan 17;11(2):461-471. doi: 10.1039/d2bm01200f.

引用本文的文献

1
Smart Biomaterials for Delivery of Drugs and Cells.用于药物和细胞递送的智能生物材料。
Biomater Res. 2025 Jul 31;29:0227. doi: 10.34133/bmr.0227. eCollection 2025.

本文引用的文献

1
Trends in mechanobiology guided tissue engineering and tools to study cell-substrate interactions: a brief review.机械生物学指导的组织工程趋势及细胞-基质相互作用研究工具：简要综述
Biomater Res. 2023 Jun 1;27(1):55. doi: 10.1186/s40824-023-00393-8.
2
An overview of in vitro 3D models of the blood-brain barrier as a tool to predict the in vivo permeability of nanomedicines.血脑屏障的体外3D模型作为预测纳米药物体内通透性工具的概述
Adv Drug Deliv Rev. 2023 May;196:114816. doi: 10.1016/j.addr.2023.114816. Epub 2023 Mar 30.
3
Human mini-blood-brain barrier models for biomedical neuroscience research: a review.
用于生物医学神经科学研究的人类微型血脑屏障模型：综述
Biomater Res. 2022 Dec 16;26(1):82. doi: 10.1186/s40824-022-00332-z.
4
TNF-α and IL-1β Modulate Blood-Brain Barrier Permeability and Decrease Amyloid-β Peptide Efflux in a Human Blood-Brain Barrier Model.TNF-α 和 IL-1β 调节血脑屏障通透性并降低人血脑屏障模型中淀粉样β肽的外排。
Int J Mol Sci. 2022 Sep 6;23(18):10235. doi: 10.3390/ijms231810235.
5
The role of cellular traction forces in deciphering nuclear mechanics.细胞牵引力在解析核力学中的作用。
Biomater Res. 2022 Sep 8;26(1):43. doi: 10.1186/s40824-022-00289-z.
6
Biomarker of Neuroinflammation in Parkinson's Disease.帕金森病神经炎症的生物标志物。
Int J Mol Sci. 2022 Apr 8;23(8):4148. doi: 10.3390/ijms23084148.
7
A Bioprinted Tubular Intestine Model Using a Colon-Specific Extracellular Matrix Bioink.使用结肠特异性细胞外基质生物墨水的生物打印管状肠模型。
Adv Healthc Mater. 2022 Jan;11(2):e2101768. doi: 10.1002/adhm.202101768. Epub 2021 Nov 16.
8
Functional angiogenesis requires microenvironmental cues balancing endothelial cell migration and proliferation.功能性血管生成需要微环境线索来平衡内皮细胞的迁移和增殖。
Lab Chip. 2020 Mar 17;20(6):1153-1166. doi: 10.1039/c9lc01170f.
9
Biomanufacturing of a novel in vitro biomimetic blood-brain barrier model.新型体外仿生血脑屏障模型的生物制造。
Biofabrication. 2020 Apr 22;12(3):035008. doi: 10.1088/1758-5090/ab4647.
10
A bioprinted human-glioblastoma-on-a-chip for the identification of patient-specific responses to chemoradiotherapy.用于鉴定患者对放化疗的特异性反应的生物打印人脑胶质瘤芯片。
Nat Biomed Eng. 2019 Jul;3(7):509-519. doi: 10.1038/s41551-019-0363-x. Epub 2019 Mar 18.