Jin Zheng, Huang Guodong, Song Yang, Zheng Xin, Zhao Kai
Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou Key Laboratory of Biomedicine and Advanced Dosage Forms, School of Life Sciences, Taizhou University, Zhejiang, Taizhou 318000, China.
Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou Key Laboratory of Biomedicine and Advanced Dosage Forms, School of Life Sciences, Taizhou University, Zhejiang, Taizhou 318000, China; Department of Biology and Medicine, College of Life Science and Medicine, Zhejiang Sci-Tech University, Zhejiang, Hangzhou 310018, China.
Carbohydr Polym. 2025 Feb 15;350:123024. doi: 10.1016/j.carbpol.2024.123024. Epub 2024 Nov 20.
Using new materials to improve detection accuracy and efficiency is important to broaden the application of immunoassay sensors. Among numerous materials for improving sensors, chitosan, as a natural polysaccharide, has excellent biocompatibility, mechanical adjustability, stimulation sensitivity and porous structure, which holds significant potential for enhancing the performance of immunoassay sensors. However, at present, there are few systematic reviews on the application of chitosan in immune sensors. In this paper, the principle of immunoassay is discussed systematically, we reviewed the recent development of chitosan enhancement strategies in various immunoassay sensors, including surface plasmon resonance immunoassay sensors, colorimetric immunoassay sensors, electrochemical immunoassay sensor, electrochemical luminescence immunoassay sensors are reviewed. Focused on the theoretical basis of improving sensor performance in immunoassay by use chitosan, as well as the various functions and applications of chitosan, and discussed how to solve the challenges of immunoassay sensors by using chitosan and the future research trend. By providing a robust foundation for the development of more efficient detection platforms, it provides insights for advancing the use of chitosan in the detection of complex biological samples. This is crucial for promoting the widespread application of immunoassay sensors with high performance in clinical diagnosis, environmental monitoring and food safety.
使用新材料提高检测准确性和效率对于拓宽免疫分析传感器的应用至关重要。在众多用于改进传感器的材料中，壳聚糖作为一种天然多糖，具有优异的生物相容性、机械可调性、刺激敏感性和多孔结构，在提升免疫分析传感器性能方面具有巨大潜力。然而，目前关于壳聚糖在免疫传感器中应用的系统综述较少。本文系统地讨论了免疫分析原理，综述了壳聚糖在各种免疫分析传感器中的增强策略的最新进展，包括表面等离子体共振免疫分析传感器、比色免疫分析传感器、电化学免疫分析传感器、电化学发光免疫分析传感器。重点阐述了利用壳聚糖提高免疫分析中传感器性能的理论基础，以及壳聚糖的各种功能和应用，并探讨了如何利用壳聚糖解决免疫分析传感器面临的挑战以及未来的研究趋势。通过为开发更高效的检测平台提供坚实基础，为推动壳聚糖在复杂生物样品检测中的应用提供了见解。这对于促进高性能免疫分析传感器在临床诊断、环境监测和食品安全中的广泛应用至关重要。
