Kamberi Blerim, Bajrami Donika, Stavileci Miranda, Omeragiq Shuhreta, Dragidella Fatmir, Koçani Ferit
Department of Dental Pathology and Endodontics, University Dentistry Clinical Center of Kosovo, 10000 Prishtina, Kosovo.
ISRN Dent. 2012;2012:390526. doi: 10.5402/2012/390526. Epub 2012 Aug 1.
Aim. The purpose of this in vitro study was to assess the antimicrobial efficacy of Biopure MTAD against E. faecalis in contaminated root canals. Materials and Methods. Forty-two single rooted extracted human teeth were inoculated with E. faecalis and incubated for four weeks. The samples were divided in two control and five experimental groups irrigated with 1.5% sodium hypochlorite solution (NaOCl); 3% NaOCl; BioPure MTAD; 1.5% NaOCl/17% EDTA; or 3% NaOCl/17% EDTA. After a one-week incubation, complete disinfection was confirmed by the absence of turbidity in the incubation media. Dentin shavings were taken from samples with no turbidity to verify whether E. faecalis was present in dentin tubules. Results were analyzed statistically using Fisher's exact test, with the level of significance set at P < 0.05. Results. Statistical analysis of the data obtained at Day 7 and after dentin shaving analysis showed that BioPure MTAD had significantly greater antibacterial activity than 1.5% NaOCl, 1.5% NaOCl/17% EDTA and 3% NaOCl/17% EDTA. No significant difference was detected between MTAD and 3% NaOCl. Conclusions. These findings suggest that BioPure MTAD possesses superior bactericidal activity compared with NaOCl and EDTA against E. faecalis.
目的。本体外研究的目的是评估碧兰MTAD（Biopure MTAD）对污染根管内粪肠球菌的抗菌效果。材料与方法。将42颗单根拔除的人牙接种粪肠球菌并培养4周。样本分为两个对照组和五个实验组，分别用1.5%次氯酸钠溶液（NaOCl）、3% NaOCl、碧兰MTAD、1.5% NaOCl/17%乙二胺四乙酸（EDTA）或3% NaOCl/17% EDTA冲洗。培养一周后，通过培养液中无浑浊来确认完全消毒。从无浑浊的样本中获取牙本质碎屑，以验证牙本质小管中是否存在粪肠球菌。使用Fisher精确检验对结果进行统计学分析，显著性水平设定为P < 0.05。结果。对第7天获得的数据以及牙本质碎屑分析后的统计分析表明，碧兰MTAD的抗菌活性显著高于1.5% NaOCl、1.5% NaOCl/17% EDTA和3% NaOCl/17% EDTA。MTAD与3% NaOCl之间未检测到显著差异。结论。这些研究结果表明，与NaOCl和EDTA相比，碧兰MTAD对粪肠球菌具有更强的杀菌活性。
