Delfan Negar, Moghadam Maryam Dalaei, Shakib Pegah, Sepahdar Asma, Naghibeiranvand Zahra
Razi Herbal Medicines Research Center, Student Research Committee, School of Dentistry, Lorestan University of Medical Sciences, Khorramabad, Iran.
Razi Herbal Medicines Research Center, Department of Endodontics, School of Dentistry, Lorestan University of Medical Sciences, Khorramabad, Iran.
Recent Pat Biotechnol. 2024 Dec 18. doi: 10.2174/0118722083342082241129060918.
Among the bioactive isoflavones identified from different plants is formononetin. Formononetin's antioxidant, anti-inflammatory, and anti-cancer qualities have all received a lot of attention lately. The goal of the current investigation was to examine formononetin's antifungal and antibacterial activity against Candida albicans and Enterococcus faecalis in vitro, respectively.
The present study determined the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and Minimum Fungicidal Concentration (MFC) of formononetin, chlorhexidine, formononetin + chlorhexidine, Formononetin+ nystatin, and nystatin against E. faecalis and C. albicans isolates. Next, SPSS version 25 was used to analyze the data. A significance cutoff of P<0.05 was taken into account.
Chlorhexidine + formononetin and formononetin alone had the greatest and lowest minimum inhibitory concentrations (MIC) against E. faecalis, at 6.6 and 18.3 μg/ml, respectively. With MIC values of 0.66 and 8.3 μg/ml against a C. albicans isolate sensitive to nystatin, the combination of formononetin + nystatin and formononetin alone was shown to have the greatest and lowest anti-candida effects.
The findings demonstrated a considerably greater antibacterial effect (P<0.05) for the combinations of formononetin + nystatin and chlorhexidine as compared to nystatin and chlorhexidine alone.
芒柄花黄素是从不同植物中鉴定出的具有生物活性的异黄酮之一。芒柄花黄素的抗氧化、抗炎和抗癌特性最近都受到了广泛关注。本研究的目的是分别检测芒柄花黄素对白色念珠菌和粪肠球菌的体外抗真菌和抗菌活性。
本研究测定了芒柄花黄素、氯己定、芒柄花黄素+氯己定、芒柄花黄素+制霉菌素和制霉菌素对粪肠球菌和白色念珠菌分离株的最低抑菌浓度（MIC）、最低杀菌浓度（MBC）和最低杀真菌浓度（MFC）。接下来，使用SPSS 25版对数据进行分析。显著性临界值取P<0.05。
氯己定+芒柄花黄素和单独的芒柄花黄素对粪肠球菌的最低抑菌浓度（MIC）最高和最低，分别为6.6和18.3μg/ml。芒柄花黄素+制霉菌素和单独的芒柄花黄素对一株对制霉菌素敏感的白色念珠菌的MIC值分别为0.66和8.3μg/ml，显示出最大和最小的抗念珠菌作用。
研究结果表明，与单独的制霉菌素和氯己定相比，芒柄花黄素+制霉菌素和氯己定组合的抗菌效果显著更强（P<0.05）。
