Sanno Mei, Kusumoto Junya, Terashi Hiroto, Sakakibara Shunsuke
Department of Plastic Surgery, Kobe University Graduate School of Medicine, Kobe, JPN.
Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, Kobe, JPN.
Cureus. 2024 Nov 29;16(11):e74775. doi: 10.7759/cureus.74775. eCollection 2024 Nov.
Nanobubbles are studied for their unique properties and possible applications in wound healing processes. This study investigates the effects of hydrogen (H₂), oxygen (O₂), and ozone (O₃) nanobubbles on fibroblast migration and proliferation using scratch wound healing assays. Fibroblast cells were treated with Dulbecco's Modified Eagle Medium (DMEM) combined with nanobubble solutions, and cell density was measured at 24 and 48 hours. While no significant difference was observed at 24 hours (p=0.52), ozone nanobubbles significantly reduced cell density at 48 hours (p=0.005), indicating cytotoxic effects. Hydrogen and oxygen nanobubble treatments did not show statistically significant differences from the control. These results highlight the cytotoxic effects of ozone nanobubbles on fibroblasts, which may impact their potential application in wound healing. While the study shows the cytotoxic effects of ozone nanobubbles, wound healing and antimicrobial impacts remain unexplored and warrant further study.
纳米气泡因其独特的性质以及在伤口愈合过程中的潜在应用而受到研究。本研究使用划痕伤口愈合试验，研究了氢气（H₂）、氧气（O₂）和臭氧（O₃）纳米气泡对成纤维细胞迁移和增殖的影响。将成纤维细胞用杜尔贝科改良 Eagle 培养基（DMEM）与纳米气泡溶液联合处理，并在 24 小时和 48 小时测量细胞密度。虽然在 24 小时时未观察到显著差异（p = 0.52），但臭氧纳米气泡在 48 小时时显著降低了细胞密度（p = 0.005），表明具有细胞毒性作用。氢气和氧气纳米气泡处理与对照组相比未显示出统计学上的显著差异。这些结果突出了臭氧纳米气泡对成纤维细胞的细胞毒性作用，这可能会影响其在伤口愈合中的潜在应用。虽然该研究显示了臭氧纳米气泡的细胞毒性作用，但其对伤口愈合和抗菌的影响仍未得到探索，值得进一步研究。
