Borges Gabriel Álvares, Elias Silvia Taveira, da Silva Sandra Márcia Mazutti, Magalhães Pérola Oliveira, Macedo Sergio Bruzadelli, Ribeiro Ana Paula Dias, Guerra Eliete Neves Silva
Faculty of Health Sciences, University of Brasília, Brasília, Brazil.
Faculty of Health Sciences, University of Brasília, Brasília, Brazil.
J Craniomaxillofac Surg. 2017 Mar;45(3):364-370. doi: 10.1016/j.jcms.2017.01.005. Epub 2017 Jan 10.
Although ozone therapy is extensively applied when wound repair and antimicrobial effect are necessary, little is known about cellular mechanisms regarding this process. Thus, this study aimed to evaluate ozone cytotoxicity in fibroblasts (L929) and keratinocytes (HaCaT) cell lines, its effects on cell migration and its antimicrobial activity. Cells were treated with ozonated phosphate-buffered saline (8, 4, 2, 1, 0.5 and 0.25 μg/mL ozone), chlorhexidine 0.2% or buffered-solution, and cell viability was determined through MTT assay. The effect of ozone on cell migration was evaluated through scratch wound healing and transwell migration assays. The minimum inhibitory concentrations for Candida albicans and Staphylococcus aureus were determined. Ozone showed no cytotoxicity for the cell lines, while chlorhexidine markedly reduced cell viability. Although no significant difference between control and ozone-treated cells was observed in the scratch assay, a considerable increase in fibroblasts migration was noticed on cells treated with 8 μg/mL ozonated solution. Ozone alone did not inhibit growth of microorganisms; however, its association with chlorhexidine resulted in antimicrobial activity. This study confirms the wound healing and antimicrobial potential of ozone therapy and presents the need for studies to elucidate the molecular mechanisms through which it exerts such biological effects.
尽管在伤口修复和抗菌作用必要时臭氧疗法被广泛应用，但关于这一过程的细胞机制却知之甚少。因此，本研究旨在评估臭氧对成纤维细胞（L929）和角质形成细胞（HaCaT）细胞系的细胞毒性、其对细胞迁移的影响及其抗菌活性。细胞用臭氧处理过的磷酸盐缓冲盐水（8、4、2、1、0.5和0.25μg/mL臭氧）、0.2%洗必泰或缓冲溶液处理，通过MTT法测定细胞活力。通过划痕伤口愈合和Transwell迁移试验评估臭氧对细胞迁移的影响。测定白色念珠菌和金黄色葡萄球菌的最低抑菌浓度。臭氧对细胞系未显示出细胞毒性，而洗必泰显著降低了细胞活力。尽管在划痕试验中未观察到对照细胞和臭氧处理细胞之间的显著差异，但在用8μg/mL臭氧处理溶液处理的细胞上，成纤维细胞迁移有相当大的增加。单独的臭氧不抑制微生物生长；然而，它与洗必泰联合使用时具有抗菌活性。本研究证实了臭氧疗法的伤口愈合和抗菌潜力，并提出需要开展研究以阐明其发挥此类生物学效应的分子机制。
