化合物柠檬醛和香叶醇的性质：体外活性和细胞毒性

Properties of Compounds Citral and Geraniol on : Activity in Vitro and Cytotoxicity.

作者信息

Baccega Bruna, Fenalti Juliana Montelli, de Mello Alexia Brauner, Islabão Yan Wahast, Birmann Paloma T, Neis Alessandra, de Giacometi Marjorie, Monteiro Francielle Liz, de Oliveira Hubner Silvia, de Almeida Vaucher Rodrigo, Farias Nara Amélia da Rosa, Norouzi Roghayeh, Siyadatpanah Abolghasem, Oliveira Camila Belmonte

机构信息

Parasitology Laboratory, Department of Microbiology and Parasitology, Federal University of Pelotas, Pelotas, Brazil.

Neurobiotechnology Research Group, Postgraduate Program in Biotechnology, Technological Development Center, UFPel, Brazil.

出版信息

Iran J Parasitol. 2024 Oct-Dec;19(4):440-447. doi: 10.18502/ijpa.v19i4.17164.

DOI:10.18502/ijpa.v19i4.17164

PMID:39735836

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11671819/

Abstract

BACKGROUND

Avian trichomoniasis is an important disease that causes bird mortality, both wild and captive, around the world. This study evaluated the in vitro cytotoxicity, antioxidant, and antiparasitic activity of citral (3.7-Dimetil-2.6-octadienal) and geraniol (trans-3.7-Dimetil-2.6-octadien-1-ol) against trophozoites.

METHODS

In vitro assays were conducted at the Laboratory of Protozoology and Entomology (LAPEN) at the Federal University of Pelotas (UFPel), Brazil in 2019 using tests with 10 parasites and citral and geraniol at concentrations ranging from 10 to 100 μM and four controls: NC (culture medium and trophozoites), MTZ (trophozoites plus 100 μM of metronidazole), and TW (trophozoites plus vehicles used for solubilizing derivatives (0.01% Tween).

RESULTS

The citral (60 μM) and geraniol (50 μM) concentrations reduced the trophozoites's viability by 100%. The molecular docking experiment demonstrated that citral and geraniol might inhibit a hydrogen enzyme for survival.

CONCLUSION

The major compounds of lemongrass have potential antitrichomonal activity against in vitro.

摘要

背景

禽毛滴虫病是一种重要疾病，在全球范围内导致野生和圈养鸟类死亡。本研究评估了柠檬醛（3,7-二甲基-2,6-辛二烯醛）和香叶醇（反式-3,7-二甲基-2,6-辛二烯-1-醇）对滋养体的体外细胞毒性、抗氧化和抗寄生虫活性。

方法

2019年在巴西佩洛塔斯联邦大学（UFPel）的原生动物学和昆虫学实验室（LAPEN）进行体外试验，使用10个寄生虫样本以及浓度范围为10至100μM的柠檬醛和香叶醇进行测试，并设置四个对照组：NC（培养基和滋养体）、MTZ（滋养体加100μM甲硝唑）和TW（滋养体加用于溶解衍生物的载体（0.01%吐温））。