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对应于几种tRNA种类的引物结合位点存在于同一类逆转录病毒样基因家族(VL30)不同成员的DNA中。

Primer binding sites corresponding to several tRNA species are present in DNAs of different members of the same retrovirus-like gene family (VL30).

作者信息

Itin A, Keshet E

出版信息

J Virol. 1985 Apr;54(1):236-9. doi: 10.1128/JVI.54.1.236-239.1985.

DOI:10.1128/JVI.54.1.236-239.1985
PMID:3973981
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC254784/
Abstract

We analyzed the putative tRNA primer binding site (PBS) present in several cloned copies of the murine retrovirus-like VL30 family. In the five VL30 DNA clones analyzed, we identified PBS sequences corresponding to three different tRNA species: tRNAPro, tRNAGly, and tRNAGln. The latter two PBS sequences have not been previously encountered in other retroviral or retrovirus-like systems. A unique situation was observed in which PBS sequences complementary to two different tRNA species were flanked by otherwise identical VL30 sequences. In addition, we demonstrated the use of PBS-specific synthetic oligonucleotides for the identification of the tRNA primer and their potential utility in the direct cloning of PBS-containing DNA elements.

摘要

我们分析了鼠类逆转录病毒样VL30家族几个克隆拷贝中存在的假定tRNA引物结合位点(PBS)。在所分析的五个VL30 DNA克隆中,我们鉴定出了对应于三种不同tRNA种类的PBS序列:tRNAPro、tRNAGly和tRNAGln。后两种PBS序列在其他逆转录病毒或逆转录病毒样系统中未曾见过。我们观察到一种独特的情况,即与两种不同tRNA种类互补的PBS序列两侧是其他方面相同的VL30序列。此外,我们证明了使用PBS特异性合成寡核苷酸来鉴定tRNA引物及其在直接克隆含PBS的DNA元件中的潜在用途。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1029/254784/edc935b0cb0c/jvirol00121-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1029/254784/edc935b0cb0c/jvirol00121-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1029/254784/edc935b0cb0c/jvirol00121-0248-a.jpg

相似文献

1
Primer binding sites corresponding to several tRNA species are present in DNAs of different members of the same retrovirus-like gene family (VL30).对应于几种tRNA种类的引物结合位点存在于同一类逆转录病毒样基因家族(VL30)不同成员的DNA中。
J Virol. 1985 Apr;54(1):236-9. doi: 10.1128/JVI.54.1.236-239.1985.
2
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本文引用的文献

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Compilation of tRNA sequences.转运RNA序列的汇编。
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Nucleotide sequence analysis of the long terminal repeat of murine virus-like DNA (VL30) and its adjacent sequences: resemblance to retrovirus proviruses.鼠类病毒样DNA(VL30)长末端重复序列及其相邻序列的核苷酸序列分析:与逆转录病毒前病毒的相似性。
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