Szabo S, Trier J S, Brown A, Schnoor J, Homan H D, Bradford J C
J Pharmacol Methods. 1985 Feb;13(1):59-66. doi: 10.1016/0160-5402(85)90068-3.
Evaluation of acute gastric erosions and ulcers induced by chemicals such as ethanol or aspirin is difficult because these lesions are predominantly multiple and irregularly shaped. A commercially available, relatively inexpensive microprocessor-linked planimeter (Micro-Plan II) with a stereomicroscope was used to measure the area of gastric mucosa damaged following intragastric administration of various doses of ethanol or aspirin. An enlarged image of rat glandular stomach was projected from the stereomicroscope onto a planimeter on which the perimeter of the entire glandular stomach and each lesion was traced with an electronic cursor. An attached printer recorded the area of stomach assessed, the number of lesions, the surface area of each lesion and the total area of mucosal damage. The technique was compared with semiquantitative methods used previously to assess experimentally induced gastric mucosal damage. The quantitative morphometric method was sufficiently sensitive to demonstrate a dose-response effect of ethanol and aspirin. The stereomicroscope--planimeter combination provides a sensitive method to quantitate either a few small, or numerous large, regularly or irregularly shaped hemorrhagic and nonhemorrhagic gastric mucosal lesions.
评估由乙醇或阿司匹林等化学物质引起的急性胃糜烂和溃疡很困难，因为这些病变主要是多发性的且形状不规则。使用一种市售的、相对便宜的与微处理器相连的面积测量仪（Micro-Plan II）和体视显微镜来测量在胃内给予各种剂量的乙醇或阿司匹林后受损胃黏膜的面积。将大鼠腺胃的放大图像从体视显微镜投射到面积测量仪上，用电子光标描绘整个腺胃和每个病变的周长。附带的打印机记录评估的胃面积、病变数量、每个病变的表面积以及黏膜损伤的总面积。将该技术与先前用于评估实验性诱导胃黏膜损伤的半定量方法进行比较。定量形态测量方法足够灵敏，能够证明乙醇和阿司匹林的剂量反应效应。体视显微镜 - 面积测量仪组合提供了一种灵敏的方法，可对少量小的或大量大的、形状规则或不规则的出血性和非出血性胃黏膜病变进行定量。
