Kowalska Małgorzata, Tylicka Marzena, Koper-Lenkiewicz Olga Martyna, Kamińska Joanna, Dorf Justyna, Matuszczak Ewa
Department of Pediatric Surgery and Urology, Medical University of Bialystok, Waszyngtona 17, 15-274, Bialystok, Poland.
Department of Biophysics, Medical University of Bialystok, Mickiewicza 2a, 15-089, Bialystok, Poland.
Sci Rep. 2025 Jan 2;15(1):474. doi: 10.1038/s41598-024-84214-5.
Cryptorchidism, the absence of one or both testicles in the scrotum, is the most common anomaly of genitourinary tract in males. The pathogenesis of undescended testes is unknown however the role of inflammation, tissue remodeling and oxidative stress is postulated. The aim of this study was to explore the role of HSP-70, MMP-3, MMP-17 and IL-8 in possible pathogenic pathways connected with cryptorchidism. Cryptorchid patients with high scrotal and inguinal canal localization were included. The study group consisted of 63 patients aged 12-24 months, whereas the control group included 17 healthy infants with the same demographic characteristics. The analysis of plasma samples was carried out using ELISA enzyme-linked immunosorbent assay technique. The plasma concentration of MMP-17 in boys with cryptorchidism was significantly higher compared to the control group (p = 0.0365). The concentration of plasma IL-8, MMP-3, and HSP-70 in boys with cryptorchidism increased in comparison to the control group but these increases were not statistically significant (p > 0.05). A positive correlation was found between plasma MMP-17 and HSP-70 concentration (r = 0.5153; p < 0.0001). Our results demonstrate that MMP-17 and HSP-70 might be involved in pathogenesis of cryptorchidism and are in line with current knowledge that high temperature has a negative influence on male gonads.
隐睾症是指阴囊内一侧或双侧睾丸缺失，是男性最常见的泌尿生殖道异常。隐睾症的发病机制尚不清楚，但推测炎症、组织重塑和氧化应激起了作用。本研究的目的是探讨热休克蛋白70（HSP-70）、基质金属蛋白酶3（MMP-3）、基质金属蛋白酶17（MMP-17）和白细胞介素8（IL-8）在与隐睾症相关的可能致病途径中的作用。纳入阴囊高位和腹股沟管型隐睾患者。研究组由63例年龄在12至24个月的患者组成，而对照组包括17例具有相同人口统计学特征的健康婴儿。采用酶联免疫吸附测定（ELISA）技术对血浆样本进行分析。与对照组相比，隐睾症男孩血浆中MMP-17的浓度显著升高（p = 0.0365）。与对照组相比，隐睾症男孩血浆中IL-8、MMP-3和HSP-70的浓度有所升高，但这些升高无统计学意义（p > 0.05）。血浆MMP-17与HSP-70浓度之间存在正相关（r = 0.5153；p < 0.0001）。我们的结果表明，MMP-17和HSP-70可能参与了隐睾症的发病机制，这与当前高温对男性性腺有负面影响的认识一致。
