Saini Aastha, Ahluwalia Kanwaljit Kaur, Ahluwalia Amrik Singh, Thakur Neelam, Negi Puneet, Hashem Abeer, Almutairi Khalid F, Abd Allah Elsayed Fathi
Department of Zoology, Akal College of Basic Sciences, Eternal University, Baru Sahib, Sirmaur 173101, Himachal Pradesh, India.
Department of Botany, Akal College of Basic Sciences, Eternal University, Baru Sahib, Sirmaur 173101, Himachal Pradesh, India.
Toxics. 2024 Nov 29;12(12):871. doi: 10.3390/toxics12120871.
Titanium dioxide (TiO) nanoparticles are being extensively used in a wide range of industrial applications for producing a variety of different consumer products, including medicines and even food items. The consumption of these products is increasing at an alarming rate, and this results in the release of these nanoparticles in the environment, causing a threat to organisms thriving in aquatic as well as terrestrial ecosystems. That is why screening such materials for their genotoxic effects, if any, becomes essential. A toxicity assay was performed to determine the LD of these nanoparticles for the mosquito by Probit analysis. Early fourth instar larvae were exposed to the selected dose of 50 µg/mL, which is <LD value, for 24 h treatment. Chromosomal slides were prepared from lacto-aceto-orcein-stained gonads of adult mosquitoes developed from treated and control larvae. These nanoparticles were reported cytotoxic as a statistically significant decline in mitotic index in treated mosquitoes than controls were observed. The nanoparticles were also found to induce various structural and numerical chromosomal aberrations in the treated lot. In the end, it can be concluded that these nanoparticles do have a genotoxic effect. The present study provides a caution against further use of these nanoparticles. There must be the development of strategies for the safe, sustainable use as well as proper disposal of these nanoparticles so as to protect both biotic and non-biotic components of the environment.
二氧化钛（TiO）纳米颗粒正广泛应用于众多工业领域，用于生产各种不同的消费品，包括药品甚至食品。这些产品的消费量正以惊人的速度增长，这导致这些纳米颗粒释放到环境中，对水生和陆地生态系统中的生物构成威胁。这就是为什么筛选此类材料是否具有遗传毒性作用变得至关重要。通过概率分析进行了毒性试验，以确定这些纳米颗粒对蚊子的半数致死剂量（LD）。将早期四龄幼虫暴露于选定的50 µg/mL剂量下，该剂量低于LD值，处理24小时。从经处理和对照幼虫发育而来的成年蚊子经乳酸-醋酸-orcein染色的性腺制备染色体玻片。据报道，这些纳米颗粒具有细胞毒性，因为观察到处理组蚊子的有丝分裂指数与对照组相比有统计学意义的下降。还发现这些纳米颗粒在处理组中诱导了各种结构和数量上的染色体畸变。最后，可以得出结论，这些纳米颗粒确实具有遗传毒性作用。本研究为进一步使用这些纳米颗粒提供了警示。必须制定安全、可持续使用以及妥善处置这些纳米颗粒的策略，以保护环境的生物和非生物成分。
