Iarema Polina, Kotovskaya Oksana, Skutel Mikhail, Drobiazko Alena, Moiseenko Andrei, Sokolova Olga, Samitova Alina, Korostin Dmitriy, Severinov Konstantin, Isaev Artem
Center for Molecular and Cellular Biology, Moscow 121205, Russia.
Faculty of Biology, Lomonosov Moscow State University, Moscow 119991, Russia.
Viruses. 2024 Dec 11;16(12):1905. doi: 10.3390/v16121905.
Using BW25113 as a host, we isolated a novel lytic phage from the commercial poly-specific therapeutic phage cocktail Sextaphage (Microgen, Russia). We provide genetic and phenotypic characterization of the phage and describe its host range on the ECOR collection of reference strains. The phage, hereafter named Sxt1, is a close relative of classical coliphage T3 and belongs to the genus, yet its internal virion proteins, forming an ejectosome, differ from those of T3. In addition, the Sxt1 lateral tail fiber (LTF) protein clusters with those of the phages from the genus. A comparison of T7, T3, and Sxt1 LTFs reveals the presence of insertions leading to the elongation of Sxt1 tail fibers, which, together with the difference in the HRDRs (host range-determining regions), might explain the expanded host specificity for the Sxt1.
以BW25113作为宿主，我们从商业化的多特异性治疗性噬菌体鸡尾酒制剂Sextaphage（俄罗斯Microgen公司）中分离出一种新型裂解性噬菌体。我们对该噬菌体进行了遗传和表型特征分析，并描述了其在ECOR参考菌株集合上的宿主范围。该噬菌体在此后命名为Sxt1，是经典大肠杆菌噬菌体T3的近亲，属于该属，但形成弹射体的内部病毒体蛋白与T3不同。此外，Sxt1的侧尾纤维（LTF）蛋白与该属噬菌体的蛋白聚在一起。对T7、T3和Sxt1的LTF进行比较发现，存在导致Sxt1尾纤维延长的插入序列，这与宿主范围决定区域（HRDRs）的差异一起，可能解释了Sxt1扩大的宿主特异性。
