Kim Dae-Sung, Cho Won-Tak, Heo Soon Chul, Huh Jung-Bo
Department of Prosthodontics, Dental Research Institute, Pusan National University Dental Hospital, Yangsan, Republic of Korea.
Department of Prosthodontics, Dental Research Institute, Dental and Life Sciences Institute, Education and Research Team for Life Science on Dentistry, School of Dentistry, Pusan National University, Yangsan, Republic of Korea.
J Adv Prosthodont. 2024 Dec;16(6):348-357. doi: 10.4047/jap.2024.16.6.348. Epub 2024 Dec 19.
This pilot study investigated the effect of surface roughness on osseointegration by comparing two types of commercial SLA-treated dental implants with different surface roughness levels: moderately rough (S = 1 - 2 µm) and rough surfaces (S > 2 µm).
Two implant groups were studied: TS (rough surface) and ADD (moderately rough surface) groups. Surface characteristics were analyzed using optical profilometry and SEM. studies using BRITER cells assessed cell adhesion, proliferation, and osteogenic differentiation through CCK-8 assay and qRT-PCR for osteopontin (OPN), osteocalcin (OCN), and alkaline phosphatase (ALP) expression. The study involved 12 implants (six per group) placed in mandibular defects of two beagle dogs. After 8 weeks, histomorphometric analysis evaluated bone to implant contact (BIC) and inter-thread bone density (ITBD). Statistical analysis used Student's t-test and two-way ANOVA for data, and Mann-Whitney U test for data.
Surface analysis revealed S values of 2.50 ± 0.27 µm for the TS group and 1.80 ± 0.06 µm for the ADD group. studies showed no significant differences in cell adhesion and proliferation between the groups ( > .05). However, gene expression patterns differed, with ADD group showing higher OPN expression ( < .001) and TS group showing higher ALP expression ( < .01). The study revealed no statistically significant differences in BIC and ITBD between the two groups ( > .05).
Surface roughness influenced osteoblast differentiation , but did not significantly affect osseointegration outcomes . Both moderately rough and rough surfaces appeared to support comparable levels of osseointegration. Larger studies are needed to confirm these findings and determine optimal implant surface characteristics.
本前瞻性研究通过比较两种具有不同表面粗糙度水平的商用SLA处理牙科种植体，研究表面粗糙度对骨结合的影响：中等粗糙度（S = 1 - 2 µm）和粗糙表面（S > 2 µm）。
研究了两个种植体组：TS（粗糙表面）组和ADD（中等粗糙表面）组。使用光学轮廓仪和扫描电子显微镜分析表面特征。使用BRITER细胞的研究通过CCK - 8测定法以及骨桥蛋白（OPN）、骨钙素（OCN）和碱性磷酸酶（ALP）表达的qRT - PCR评估细胞粘附、增殖和成骨分化。该研究涉及将12枚种植体（每组6枚）植入两只比格犬的下颌骨缺损处。8周后，组织形态计量学分析评估骨与种植体接触（BIC）和螺纹间骨密度（ITBD）。统计分析对计量数据使用学生t检验和双向方差分析，对计数数据使用曼 - 惠特尼U检验。
表面分析显示TS组的S值为2.50 ± 0.27 µm，ADD组为1.80 ± 0.06 µm。细胞研究表明两组之间在细胞粘附和增殖方面无显著差异（P > 0.05）。然而，基因表达模式不同，ADD组显示较高的OPN表达（P < 0.001），TS组显示较高的ALP表达（P < 0.01）。组织形态计量学研究表明两组之间在BIC和ITBD方面无统计学显著差异（P > 0.05）。
表面粗糙度影响成骨细胞分化，但对骨结合结果无显著影响。中等粗糙度和粗糙表面似乎都支持相当水平的骨结合。需要更大规模的研究来证实这些发现并确定最佳种植体表面特征。
