耐盐酵母汉逊德巴利酵母中的甘油代谢与渗透调节
Glycerol metabolism and osmoregulation in the salt-tolerant yeast Debaryomyces hansenii.
作者信息
Adler L, Blomberg A, Nilsson A
出版信息
J Bacteriol. 1985 Apr;162(1):300-6. doi: 10.1128/jb.162.1.300-306.1985.
Abstract
A glycerol-nonutilizing mutant of the salt-tolerant yeast Debaryomyces hansenii was isolated. When subjected to salt stress the mutant produced glycerol, and the internal level of glycerol increased linearly in proportion to increases of external salinity as in the wild-type strain. However, at increased salinity the mutant showed a more pronounced decrease of growth rate and growth yield and lost more glycerol to the surrounding medium than did the wild type. Uptake experiments showed glycerol to be accumulated against a strong concentration gradient, and both strains displayed similar kinetic parameters for the uptake of glycerol. An examination of enzyme activities of the glycerol metabolism revealed that the apparent Km of the sn-glycerol 3-phosphate dehydrogenase (EC 1.1.99.5) was increased 330-fold for sn-glycerol 3-phosphate in the mutant. Based on the findings, a scheme for the pathways of glycerol metabolism is suggested.
摘要
分离得到了耐盐酵母汉逊德巴利酵母的一个甘油非利用突变体。当受到盐胁迫时，该突变体产生甘油，并且其内部甘油水平与野生型菌株一样，随着外部盐度的增加呈线性增加。然而，在盐度增加时，该突变体的生长速率和生长产量下降更为明显，并且比野生型向周围培养基中损失更多的甘油。摄取实验表明甘油是逆着强浓度梯度积累的，并且两种菌株在甘油摄取方面表现出相似的动力学参数。对甘油代谢酶活性的检查发现，突变体中sn-甘油-3-磷酸脱氢酶（EC 1.1.99.5）对sn-甘油-3-磷酸的表观Km增加了330倍。基于这些发现，提出了甘油代谢途径的示意图。
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本文引用的文献
1
The activation of glycerol dehydrogenase from Aerobacter aerogenes by monovalent cations.产气气杆菌甘油脱氢酶的一价阳离子激活作用。
J Biol Chem. 1960 Jun;235:1820-3.
2
Utilization of L-alpha-glycerophosphate by Escherichia coli without hydrolysis.大肠杆菌对L-α-甘油磷酸的非水解利用。
Proc Natl Acad Sci U S A. 1962 Dec 15;48(12):2145-50. doi: 10.1073/pnas.48.12.2145.
3
alpha-Glycerophosphate oxidase of flight muscle mitochondria.飞行肌线粒体的α-甘油磷酸氧化酶
J Biol Chem. 1958 Oct;233(4):1014-9.
4
Kinetics of glycerol kinases from mammalian liver and Candida mycoderma.哺乳动物肝脏和假丝酵母甘油激酶的动力学
Eur J Biochem. 1967 Dec;3(1):78-84. doi: 10.1111/j.1432-1033.1967.tb19500.x.
5
Glycerol metabolism in yeasts. Pathways of utilization and production.酵母中的甘油代谢。利用和产生途径。
Eur J Biochem. 1968 Jul;5(2):165-72. doi: 10.1111/j.1432-1033.1968.tb00353.x.
6
Localization of the glycerol-phosphate dehydrogenase in the outer phase of the mitochondrial inner membrane.磷酸甘油脱氢酶在线粒体内膜外相的定位。
Eur J Biochem. 1970 Apr;13(2):247-52. doi: 10.1111/j.1432-1033.1970.tb00924.x.
7
Regulation of the potassium to sodium ratio and of the osmotic potential in relation to salt tolerance in yeasts.酵母中钾钠比及渗透势与耐盐性的关系调控
J Bacteriol. 1969 Nov;100(2):836-45. doi: 10.1128/jb.100.2.836-845.1969.
8
Feedback inhibition of glycerol kinase, a catabolic enzyme in Escherichia coli.甘油激酶的反馈抑制，大肠杆菌中的一种分解代谢酶。
Science. 1966 Aug 12;153(3737):755-7. doi: 10.1126/science.153.3737.755.
9
Growth stasis by accumulated L-alpha-glycerophosphate in Escherichia coli.大肠杆菌中累积的L-α-甘油磷酸导致生长停滞。
J Bacteriol. 1965 Nov;90(5):1325-9. doi: 10.1128/jb.90.5.1325-1329.1965.
10
Catalytic and allosteric properties of glycerol kinase from Escherichia coli.大肠杆菌甘油激酶的催化和别构特性
J Biol Chem. 1973 Jun 10;248(11):3922-32.
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