Trivanović Drenka, Vujačić Marko, Arsić Aleksandra, Kukolj Tamara, Rajković Milica, Bogosavljević Nikola, Baščarević Zoran, Maljković Ružičić Mirjana, Kovačević Jovana, Jauković Aleksandra
Group for Hematology and Stem Cells, Institute for Medical Research, National Institute of Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia.
Institute for Orthopedy Banjica, 11000 Belgrade, Serbia.
Metabolites. 2025 Jan 4;15(1):16. doi: 10.3390/metabo15010016.
BACKGROUND/OBJECTIVES: Bone marrow adipose tissue (BMAT) has been described as an important biomechanic and lipotoxic factor with negative impacts on skeletal and hematopoietic system regeneration. BMAT undergoes metabolic and cellular adaptations with age and disease, being a source of potential biomarkers. However, there is no evidence on the lipid profile and cellularity at different skeletal locations in osteoarthritis patients undergoing primary hip arthroplasty.
Acetabular and femoral bone marrow (BM) and gluteofemoral subcutaneous adipose tissue (gfSAT) were obtained from matched patients undergoing hip replacement surgery. BM, BMAT, and gfSAT were explored at the levels of total lipids, fatty acids, and cells by using thin-layerand gas chromatography, ex vivo cellular assays, and flow cytometry.
BMAT content was significantly higher in femoral than in acetabular BM. Total lipid analyses revealed significantly lower triglyceride content in femoral than in acetabular BMAT and gfSAT. Frequencies of saturated palmitic, myristic, and stearic acids were higher in femoral than in acetabular BMAT and gfSAT. The content of CD45CD34 cells within femoral BMAT was higher than in acetabular BMAT or gfSAT. This was associated with a higher incidence of total clonogenic hematopoietic progenitors and late erythroid colonies CFU-E in femoral BMAT when compared to acetabular BMAT, similar to their BM counterparts.
Collectively, our results indicate that the lipid profiles of hip bone and femoral BMAT impose significantly different microenvironments and distributions of cells with hematopoietic potential. These findings might bring forth new inputs for defining BMAT biology and setting novel directions in OA disease investigations.
背景/目的：骨髓脂肪组织（BMAT）被认为是一种重要的生物力学和脂毒性因子，对骨骼和造血系统的再生具有负面影响。BMAT会随着年龄和疾病发生代谢和细胞适应性变化，是潜在生物标志物的来源。然而，对于接受初次髋关节置换术的骨关节炎患者不同骨骼部位的脂质谱和细胞组成，尚无相关证据。
从接受髋关节置换手术的配对患者中获取髋臼和股骨骨髓（BM）以及臀股皮下脂肪组织（gfSAT）。通过薄层色谱法和气相色谱法、体外细胞试验以及流式细胞术，在总脂质、脂肪酸和细胞水平上对BM、BMAT和gfSAT进行研究。
股骨BM中的BMAT含量显著高于髋臼BM。总脂质分析显示，股骨BMAT中的甘油三酯含量显著低于髋臼BMAT和gfSAT。股骨中饱和棕榈酸、肉豆蔻酸和硬脂酸的频率高于髋臼BMAT和gfSAT。股骨BMAT中CD45CD34细胞的含量高于髋臼BMAT或gfSAT。与髋臼BMAT相比，这与股骨BMAT中总克隆造血祖细胞和晚期红系集落CFU-E的发生率较高有关，与它们的骨髓对应物相似。
总体而言，我们的结果表明，髋骨和股骨BMAT的脂质谱形成了显著不同的微环境以及具有造血潜力的细胞分布。这些发现可能为定义BMAT生物学特性和为骨关节炎疾病研究设定新方向提供新的依据。
