Leijon Mikael, Axén Charlotte, Banihashem Fereshteh, Lilja Tobias, Tibblin Petter, Persson Björn David
Swedish Veterinary Agency, 751 89 Uppsala, Sweden.
Ecology and Evolution in Microbial Model Systems, EEMiS, Department of Biology and Environmental Science, Linnaeus University, 391 82 Kalmar, Sweden.
Pathogens. 2025 Jan 8;14(1):45. doi: 10.3390/pathogens14010045.
During routine sampling of northern pike, a male with circular blue-metallic granular spots mainly located on the head and back was identified. Histological investigations presented multifocally thickened epidermis rich in basophilic large structures with a granulated rim and a dense, non-granulated center. Other organs showed no signs of infection. Ultrastructural analysis of the skin revealed three different types of herpes-like structures predominantly located within enlarged vacuoles. PCR analysis and NGS of dissected skin tissue verified the presence of EsHV1 DNA. In this study, we describe the first identification of EsHV1 in mainland Europe. In addition, for the first time, full sequences of both the DNA polymerase and terminase of the virus is available, thus allowing for an improved phylogenetic placement of EsHV1 within the family. In addition to the EsHV1 infected pike, we also observed that 11.1% of the pike were affected by lymphosarcoma, a hyperplasia-disease caused by retroviruses. In conclusion, viral infections in pike are relatively common and likely have consequences for the local population dynamics.
在对白斑狗鱼进行常规采样时，发现了一条雄性白斑狗鱼，其头部和背部主要分布着圆形的蓝色金属颗粒状斑点。组织学研究显示，表皮多处增厚，富含嗜碱性大结构，边缘呈颗粒状，中心致密、无颗粒。其他器官未显示感染迹象。对皮肤的超微结构分析揭示了三种不同类型的疱疹样结构，主要位于扩大的液泡内。对解剖后的皮肤组织进行PCR分析和NGS验证了艾氏疱疹病毒1型（EsHV1）DNA的存在。在本研究中，我们描述了在欧洲大陆首次鉴定出EsHV1。此外，首次获得了该病毒DNA聚合酶和末端酶的完整序列，从而能够更好地将EsHV1在该病毒科内进行系统发育定位。除了感染EsHV1的白斑狗鱼外，我们还观察到11.1%的白斑狗鱼受到淋巴肉瘤的影响，淋巴肉瘤是一种由逆转录病毒引起的增生性疾病。总之，白斑狗鱼中的病毒感染相对常见，可能会对当地种群动态产生影响。
