Boyce J M, Evans E J, Evans D G, DuPont H L
Infect Immun. 1979 Aug;25(2):532-7. doi: 10.1128/iai.25.2.532-537.1979.
Seven isolates of Yersinia enterocolitica serotype 0:8, recovered during an outbreak of gastrointestinal illness, were examined for enterotoxin production. All seven strains were enterotoxigenic in the suckling mouse model, and three of five isolates tested produced keratoconjunctivitis in the guinea pig eye model (Sereny test). Enterotoxin was detected in broth supernatant fluid after 12 h of incubation at 25 degrees C. The toxin was not inactivated by exposure to 121 degrees C for 30 min or by storage at 4 or -40 degrees C for at least 5 months. The enterotoxin was also acid stable and methanol soluble. Methanol extraction did not affect its heat stability or enterotoxic activity in suckling mice. These physical characteristics plus limited ultrafiltration studies suggest that the enterotoxin is a low-molecular-weight substance. Y. enterocolitica enterotoxin resembles Escherichia coli heat-stable enterotoxin in heat and pH stability, methanol solubility, and enterotoxic activity in suckling mice. However, its role in the pathogenesis of human diarrhea has not been established.
对在一次胃肠道疾病暴发期间分离出的7株小肠结肠炎耶尔森菌0:8血清型菌株进行了肠毒素产生情况检测。所有7株菌株在乳鼠模型中均产肠毒素，且在豚鼠眼模型（塞雷尼试验）中，5株受试菌株中有3株引起了角膜结膜炎。在25℃培养12小时后，在肉汤上清液中检测到了肠毒素。该毒素经121℃处理30分钟或在4℃或-40℃储存至少5个月后未失活。肠毒素也具有酸稳定性且可溶于甲醇。甲醇提取不影响其热稳定性或在乳鼠中的肠毒性活性。这些物理特性以及有限的超滤研究表明该肠毒素是一种低分子量物质。小肠结肠炎耶尔森菌肠毒素在热稳定性、pH稳定性、甲醇溶解性以及在乳鼠中的肠毒性活性方面类似于大肠杆菌热稳定肠毒素。然而，其在人类腹泻发病机制中的作用尚未明确。
