Civzele Anna, Mezule Linda
Water Systems and Biotechnology Institute, Riga Technical University, Latvia.
Biotechnol Rep (Amst). 2025 Jan 10;45:e00875. doi: 10.1016/j.btre.2025.e00875. eCollection 2025 Mar.
The study investigates the application of white rot fungi for reactor-scale microalgae harvesting and explores the mechanisms underlying the algal-fungal interactions and their impact on biomass composition. Enzymatic analysis and microscopy revealed that the formation of algal-fungal complexes and successful harvesting are coupled with fungal cellulose-degrading enzyme production and hydrolytic processes of microalgae cells. Fluorescence intensity decreased by over 80 % in cells stained with Calcofluor-white after interaction with white rot fungi, indicating the reduction in cellulose content in microalgal cells caused by fungal enzymatic activity. These enzymes also caused significant cell damage and more than 50 % decrease in microalgae cell size. The presence of cellulolytic enzymes broadens the potential application of the resulting biomass in various biotechnological applications. Moreover, reactor-scale bioflocculation resulted in over 95 % and almost 85 % harvesting efficiency from secondary wastewater within less than 24 h, demonstrating the method's scalability and industrial applicability.
该研究调查了白腐真菌在反应器规模下用于微藻收获的应用，并探索了藻菌相互作用的潜在机制及其对生物质组成的影响。酶分析和显微镜观察表明，藻菌复合物的形成和成功收获与真菌纤维素降解酶的产生以及微藻细胞的水解过程相关。与白腐真菌相互作用后，用荧光增白剂染色的细胞中荧光强度降低了80%以上，这表明真菌酶活性导致微藻细胞中纤维素含量减少。这些酶还造成了显著的细胞损伤，微藻细胞大小减小了50%以上。纤维素分解酶的存在拓宽了所得生物质在各种生物技术应用中的潜在用途。此外，在不到24小时的时间内，反应器规模的生物絮凝从二级废水中收获效率超过95%，几乎达到85%，证明了该方法的可扩展性和工业适用性。
