Wei Hehong, Gao Na, Liu Yaqi, Sun Yangyang, Dai Jingcheng, Yao Ying, Ding Bingqing, Sun Si, Geng Yamin
College of Energy and Environmental Engineering, Hebei University of Engineering, Handan, 056038, China.
Key Laboratory of Freshwater Aquaculture and Enhancement of Anhui Province, Fisheries Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, China.
Curr Microbiol. 2025 Mar 10;82(4):183. doi: 10.1007/s00284-025-04142-z.
A novel bacterial strain, designated Dechloromonas aquae ZY10, was isolated from the aquaculture water of grass carp. The colonies exhibited diameters ranging from approximately 1 to 3 mm and were characterized by a creamy-white coloration, circular shape, smooth texture, translucency, and a convex profile. The cells were facultatively anaerobic and motile, utilizing a single polar flagellum for movement. They were rod-shaped, reproduced through binary fission, and were identified as Gram-negative, oxidase-negative, and catalase-positive. Optimal growth was observed between 37 and 40 °C, within a pH range of 7.0-9.0, and at a NaCl concentration of 0% (w/v). The respiratory quinone was ubiquinone-8 (97.6%) and ubiquinone-7 (2.4%), and the major polar lipids were phosphatidylethanolamine (PE), aminophospholipid (APL), diphosphatidylglycerol (DPG), and phosphatidylglycerol (PG). Phylogenetic analysis based on 16S rRNA gene sequences suggested that ZY10 formed a lineage within the genus Dechloromonas and showed the highest 16S rRNA gene sequence similarity to "D. hankyongensis" XY25 (96.5%), followed by D. denitrificans ED1 (96.4%), D. hortensis MA-1 (96.1%) and D. agitata CKB (96.0%) and Azonexus. caeni Slo-05 (95.5%). The predominant fatty acids of ZY10 were summed feature 3 (comprising C ω6c and/or C ω7c) and C. The whole genome of ZY10 was 3,568,927 bp in size, including 3,275 protein-coding genes, 72 tRNA genes, and 15 rRNA genes, and the genomic DNA G + C content was 62.0 mol%. The orthologous average nucleotide identity, average amino acid identity, and digital DNA-DNA hybridization values between ZY10 and other species within the family Azonexaceae were 78.0-81.5%, 71.8-73.9%, and 20.3-23.0%, respectively. Therefore, based on phenotypic, chemotaxonomic, and phylogenetic analyses, the isolated ZY10 (= MCCC 1K08699 = KCTC 72749) is proposed as type strain of the novel species Dechloromonas aquae sp. nov.
从草鱼养殖水中分离出一种新型细菌菌株，命名为水脱氯单胞菌ZY10。菌落直径约为1至3毫米，特征为乳白色、圆形、质地光滑、半透明且凸起。细胞为兼性厌氧且具运动性，利用单根极鞭毛进行运动。它们呈杆状，通过二分裂繁殖，经鉴定为革兰氏阴性、氧化酶阴性和过氧化氢酶阳性。在37至40°C、pH值7.0 - 9.0以及NaCl浓度0%（w/v）的条件下观察到最佳生长。呼吸醌为泛醌 - 8（97.6%）和泛醌 - 7（2.4%），主要极性脂为磷脂酰乙醇胺（PE）、氨基磷脂（APL）、二磷脂酰甘油（DPG）和磷脂酰甘油（PG）。基于16S rRNA基因序列的系统发育分析表明，ZY10在脱氯单胞菌属内形成一个分支，与“汉京脱氯单胞菌”XY25的16S rRNA基因序列相似度最高（96.5%），其次是反硝化脱氯单胞菌ED1（96.4%）、园艺脱氯单胞菌MA - 1（96.1%）、搅动脱氯单胞菌CKB（96.0%）和新氮单胞菌属的Caeni Slo - 05（95.5%）。ZY10的主要脂肪酸为总和特征3（包含C ω6c和/或C ω7c）和C。ZY10的全基因组大小为3,568,927 bp，包括3,275个蛋白质编码基因、72个tRNA基因和15个rRNA基因，基因组DNA的G + C含量为62.0 mol%。ZY10与氮单胞菌科其他物种之间的直系同源平均核苷酸同一性、平均氨基酸同一性和数字DNA - DNA杂交值分别为78.0 - 81.5%、71.8 - 73.9%和20.3 - 23.0%。因此，基于表型、化学分类和系统发育分析，提议将分离得到的ZY10（= MCCC 1K08699 = KCTC 72749）作为新物种水脱氯单胞菌的模式菌株。
