健康人类血视网膜屏障的通透性。欧洲眼荧光测定法协同行动。

Permeability of the blood-retinal barrier in healthy humans. European Concerted Action on Ocular Fluorometry.

作者信息

Van Schaik H J, Heintz B, Larsen M, Leite E, Rosas V, Schalnus R, Van Best J A

机构信息

Department of Ophthalmology, Leiden University Medical Center, The Netherlands.

出版信息

Graefes Arch Clin Exp Ophthalmol. 1997 Oct;235(10):639-46. doi: 10.1007/BF00946940.

DOI:10.1007/BF00946940

PMID:9349948

Abstract

BACKGROUND

The aim of this study was to compare the inward permeability of the blood-retinal barrier in healthy subjects from six European cities.

METHODS

Seventy-two healthy subjects (age 20-70 years) were selected. At 30 min and 60 min after fluorescein injection, fluorescein mass in vitreous was calculated from the concentrations measured along the optical axis of the eye. Non-protein-bound fluorescein (NPBF) concentrations were measured in plasma prepared from blood samples taken 7, 15 and 55 min after injection. Blood-retinal barrier permeability (PBRB) was calculated from the vitreous fluorescein mass and the time integral of NPBF and was corrected for the autofluorescence of ocular tissue and for lenticular light transmittance.

RESULTS

Mean PBRB values +/- SD (nm.s-1) were 2.07 +/- 0.54 (Coimbra), 2.01 +/- 0.43 (Frankfurt), 2.24 +/- 0.50 (Ghent), 2.37 +/- 0.56 (Herlev), 1.89 +/- 0.44 (Leiden) and 1.74 +/- 0.38 (Porto). Differences between centers were not significant (P > 0.35). Measurements were reproducible and independent of the time after fluorescein injection (P > 0.50). A PBRB higher than 3.16 nm.s-1 or a value which had increased by 32% was considered abnormal (P < 0.05).

CONCLUSION

PBRB values were similar in all centers. The results demonstrate that this is a highly sensitive and reliable method for measuring the permeability of the blood-retinal barrier.

摘要

背景

本研究的目的是比较来自欧洲六个城市的健康受试者血视网膜屏障的内向通透性。

方法

选取72名健康受试者（年龄20 - 70岁）。在注射荧光素后30分钟和60分钟，根据沿眼光轴测量的浓度计算玻璃体内的荧光素质量。在注射后7分钟、15分钟和55分钟采集的血样制备的血浆中测量非蛋白结合荧光素（NPBF）浓度。根据玻璃体内荧光素质量和NPBF的时间积分计算血视网膜屏障通透性（PBRB），并对眼组织的自发荧光和晶状体透光率进行校正。

结果

各中心的平均PBRB值±标准差（nm·s⁻¹）分别为：科英布拉2.07±0.54、法兰克福2.01±0.43、根特2.24±0.50、赫勒夫2.37±0.56、莱顿1.89±0.44、波尔图1.74±0.38。各中心之间的差异不显著（P>0.35）。测量结果具有可重复性，且与荧光素注射后的时间无关（P>0.50）。PBRB高于3.16 nm·s⁻¹或升高32%的值被认为异常（P<0.05）。