Lee Hsiang-Ying, Lin Chung Yu, Juan Yung-Shun, Wu Wen-Jeng, Cho Sung Yong, Wu Deng-Chyang
Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.
Department of Urology, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Urolithiasis. 2025 Mar 19;53(1):53. doi: 10.1007/s00240-025-01724-1.
Urolithiasis is a disease with high prevalence and recurrence rate. There are various risk factors impacting on stone formation including intestinal micorbiota. This study aims to investigate the relationship between urine microbiota with urolithiasis. We collected mid-stream voided urine samples from urolithiasis patients and control participants and stored them in a freezer at - 80 °C. All enrolled participants were requested to provide information about their clinical characteristics. The procedure included the extraction of the genomic DNA from the urine samples; the amplification by polymerase chain reaction (PCR); PCR product quantification, mixing, and purification; DNA library preparation; and sequencing was performed with quality control (QC) measures. Alpha diversity was indicative of the species complexity within individual urine samples, and beta diversity analysis was used to evaluate the differences among the samples in terms of species complexity. We enrolled 28 urolithiasis patients and 59 control participants who reported no recent antibiotic usage. In the beta diversity analysis, there was a significant difference between the microbiota in the samples of the urolithiasis and control groups according to ANOSIM statistical analysis. (P = 0.004). On comparing the groups, it showed Alcaligenes, Bacteroides, Blautia, Ruminococcaceae_UCG, Cutibacterium, Alistipes, Lachnoclostridium present more significant in control group than urolithiasis patients. In conclusion, our current study shows that dysbiosis of urine microbiota may be related to the development of urolithiasis. Further research targeting specific microbes to identify their role in the development of diseases is necessary and might provide novel diagnostic biomarkers and therapeutic options.
尿路结石是一种发病率和复发率都很高的疾病。有多种危险因素会影响结石形成，包括肠道微生物群。本研究旨在探讨尿液微生物群与尿路结石之间的关系。我们收集了尿路结石患者和对照参与者的中段晨尿样本，并将其储存在-80°C的冰箱中。所有纳入的参与者都被要求提供他们的临床特征信息。该程序包括从尿液样本中提取基因组DNA；通过聚合酶链反应（PCR）进行扩增；PCR产物定量、混合和纯化；DNA文库制备；并进行测序以及质量控制（QC）措施。α多样性表示个体尿液样本中的物种复杂性，β多样性分析用于评估样本之间在物种复杂性方面的差异。我们纳入了28名尿路结石患者和59名未报告近期使用抗生素的对照参与者。在β多样性分析中，根据ANOSIM统计分析，尿路结石组和对照组样本中的微生物群存在显著差异（P = 0.004）。比较两组时发现，产碱杆菌属、拟杆菌属、布劳特氏菌属、瘤胃球菌科_UCG、棒状杆菌属、阿里斯杆菌属、毛螺菌属在对照组中比尿路结石患者中更为显著。总之，我们目前的研究表明，尿液微生物群失调可能与尿路结石的发生有关。针对特定微生物以确定它们在疾病发生中的作用的进一步研究是必要的，并且可能会提供新的诊断生物标志物和治疗选择。
