大肠杆菌噬菌体Môr Ffagbaw的基因组序列与特征分析
Genome Sequence and Characterization of Coliphage Môr Ffagbaw.
作者信息
Thomas Nerissa E, Ugokwe Nzubechukwu Innocent, Kinsella Andrew, Galyov Edouard, van Schaik Willem, Joyce Naomi, Nobrega Franklin L, Millard Andrew, Wilkinson Rachael C
机构信息
Swansea University Medical School, Swansea University, Swansea, United Kingdom.
Centre for Phage Research, University of Leicester, Leicester, United Kingdom.
出版信息
Phage (New Rochelle). 2023 Dec 14;4(4):159-164. doi: 10.1089/phage.2023.0029. eCollection 2023 Dec.
BACKGROUND
The quality of coastal waters around the United Kingdom is an area of increasing concern following sewer overflow, where wastewater is discharged into the environment. Coliphages, viruses that infect coliform bacteria, are associated with water quality in aquatic systems, yet remain largely uncharacterized at the genomic level.
MATERIALS AND METHODS
Phage môr ffagbaw was isolated from seawater against by enrichment and plaque assays. Whole genome sequencing, transmission electron microscopy, and host range analysis against the reference (ECOR) collection were used to characterize the phage.
RESULTS
The virion had a siphovirus morphology and genomic analysis placed it within the family , subfamily and forms a new species within the genus . Spot assays revealed that phage môr ffagbaw could form plaques on 6 out of 72 ECOR strains (8%).
CONCLUSIONS
Môr ffagbaw represents a new species of phage within the genus , with a narrow host range.
背景
在英国，随着污水溢流（即废水排放到环境中），沿海水域的水质问题日益受到关注。感染大肠菌群细菌的噬菌体与水生系统中的水质相关，但在基因组水平上仍基本未被表征。
材料与方法
通过富集和噬菌斑测定从海水中分离出噬菌体môr ffagbaw。使用全基因组测序、透射电子显微镜以及针对参考（ECOR）菌株集的宿主范围分析来表征该噬菌体。
结果
该病毒体具有长尾噬菌体形态，基因组分析将其归入长尾噬菌体科、T5样噬菌体亚科，并在T5样噬菌体属内形成一个新物种。斑点试验表明，噬菌体môr ffagbaw可在72株ECOR菌株中的6株上形成噬菌斑（8%）。
结论
Môr ffagbaw代表T5样噬菌体属内的一种新噬菌体物种，宿主范围狭窄。
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