Wu Shaowen, Feng Ke, Niu Jinlu, Xu Jintao, Mo Hualian, She Xiaoman, Yu Shang-Bo, Li Zhan-Ting, Yan Shijuan
State Key Laboratory of Swine and Poultry Breeding Industry, Agro-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
State Key Laboratory of Organometallic Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, University of Chinese Academy of Sciences, 345 Lingling Lu, Shanghai 200032, China.
Toxins (Basel). 2025 Feb 25;17(3):104. doi: 10.3390/toxins17030104.
Aflatoxin B1 (AFB1), a highly toxic secondary metabolite produced by Aspergillus species, represents a significant health hazard due to its widespread contamination of agricultural products. The urgent need for sensitive and sustainable detection methods has driven the development of diverse analytical approaches, most of which heavily rely on organic solvents, posing environmental challenges for routine food safety analysis. Here, we introduce a supramolecular platform leveraging acyclic cucurbit[n]uril (acCB) as a host molecule for environmentally sustainable AFB1 detection. Screening various acCB derivatives identified acCB6 as a superior host capable of forming a stable 1:1 complex with AFB1 in an aqueous solution, exhibiting a high binding affinity. Proton nuclear magnetic resonance (1H NMR) spectroscopy confirmed that AFB1 was deeply encapsulated within the host cavity, with isothermal titration calorimetry (ITC) experiments and molecular dynamics simulations further substantiating the stability of the interaction, driven by enthalpic and entropic contributions. This supramolecular host was incorporated into a scaffold-assembly-based bioluminescent enzyme immunoassay (SA-BLEIA), providing a green detection platform that rivals the performance of traditional organic solvent-based assays. Our findings highlight the potential of supramolecular chemistry as a foundation for eco-friendly mycotoxin detection and offer valuable insights into designing environmentally sustainable analytical methods.
黄曲霉毒素B1（AFB1）是曲霉菌种产生的一种剧毒次生代谢产物，由于其对农产品的广泛污染，对健康构成重大危害。对灵敏且可持续检测方法的迫切需求推动了多种分析方法的发展，其中大多数方法严重依赖有机溶剂，给常规食品安全分析带来了环境挑战。在此，我们介绍一种超分子平台，该平台利用无环葫芦[n]脲（acCB）作为主体分子，用于环境可持续的AFB1检测。通过筛选各种acCB衍生物，确定acCB6是一种优良的主体，能够在水溶液中与AFB1形成稳定的1:1复合物，表现出高结合亲和力。质子核磁共振（1H NMR）光谱证实AFB1被深度包裹在主体腔内，等温滴定量热法（ITC）实验和分子动力学模拟进一步证实了由焓和熵贡献驱动的相互作用的稳定性。这种超分子主体被整合到基于支架组装的生物发光酶免疫分析（SA - BLEIA）中，提供了一个绿色检测平台，其性能可与传统的基于有机溶剂的分析方法相媲美。我们的研究结果突出了超分子化学作为生态友好型霉菌毒素检测基础的潜力，并为设计环境可持续的分析方法提供了有价值的见解。
