Battah Basem, Chianese Teresa, Rosati Luigi, Petretto Giacomo, Soukkarieh Chadi, Ferrari Marco, Mazzarello Vittorio, Barac Aleksandra, Peric Aleksandar, Donadu Matthew Gavino
Department of Microbiology and Biochemistry, Antioch Syrian Private University, Maaret Saidnaya 22734, Syria.
Department of Biology, University Federico II, Via Cintia 21, 80126 Napoli, Italy.
Antibiotics (Basel). 2025 Mar 12;14(3):293. doi: 10.3390/antibiotics14030293.
Chemotherapy continues to be the cornerstone for the management of leishmaniasis. The preferred medications are pricey and have a number of unfavorable side effects. These restrictions make it necessary to produce novel antileishmanial chemicals, and plants have opportunities in this respect. This study aimed to evaluate the antileishmanial properties of essential oil and its mechanisms of action. Our findings demonstrated that essential oil, rich in thymol, exhibited potent antileishmanial activity, with an IC50 value of approximately 1 µg/mL against promastigotes. Furthermore, the cell cycle arrest at the sub-G0-G1 phase supported the theory that the leishmanicidal effect was mediated by apoptosis. The essential oil was characterized using gas chromatography-tandem mass spectrometry. Antileishmanial activity against promastigotes was assessed, with mechanisms confirmed via flow cytometry. These results confirm the potential of essential oil as a promising therapeutic candidate for the treatment of leishmaniasis.
化疗仍然是利什曼病治疗的基石。首选药物价格昂贵且有许多不良副作用。这些限制使得有必要开发新型抗利什曼药物，而植物在这方面具有潜力。本研究旨在评估精油的抗利什曼特性及其作用机制。我们的研究结果表明，富含百里香酚的精油表现出强大的抗利什曼活性，对前鞭毛体的IC50值约为1μg/mL。此外，细胞周期停滞在亚G0-G1期支持了利什曼icidal作用是由凋亡介导的理论。通过气相色谱-串联质谱法对精油进行了表征。评估了对前鞭毛体的抗利什曼活性，并通过流式细胞术确认了其作用机制。这些结果证实了精油作为治疗利什曼病的有前景的治疗候选物的潜力。
