Roberts-Thomson P J, McEvoy R, Langhans T, Bradley J
Ann Rheum Dis. 1985 Jun;44(6):379-83. doi: 10.1136/ard.44.6.379.
In a cross-sectional study of over 3000 consecutive serum specimens the levels of rheumatoid factor (RF) measured by rate nephelometry (Beckman ICS II) were compared with values obtained by the more traditional methods of sheep cell agglutination (Rose-Waaler) and latex agglutination. Similar values for sensitivity and specificity were found for all three methods for rheumatoid arthritis, with nephelometry giving slightly higher levels of sensitivity for other rheumatic disorders. A significant correlation (r = 0.46, p less than 0.01) was found between the nephelometric and Rose-Waaler method for 147 consecutive seropositive specimens. Of interest, however, several disparate results were observed, and explanations for these were sought. Longitudinal studies of RF were performed in 49 seropositive patients over a two-year period. The nephelometric method was considered superior compared with the other techniques because of its ability to detect changes in absolute levels at earlier stages and its low interassay coefficient of variance (11%). We conclude that the nephelometric technique appears suitable for routine diagnostic use, offers several advantages compared with more traditional methods, and is no more expensive per test specimen than the Rose-Waaler technique.
在一项对超过3000份连续血清标本的横断面研究中，通过速率散射比浊法（贝克曼ICS II）测定的类风湿因子（RF）水平与通过更传统的绵羊细胞凝集法（罗斯-瓦勒法）和乳胶凝集法获得的值进行了比较。对于类风湿关节炎，三种方法的敏感性和特异性值相似，散射比浊法对其他风湿性疾病的敏感性略高。在147份连续的血清阳性标本中，散射比浊法与罗斯-瓦勒法之间存在显著相关性（r = 0.46，p < 0.01）。然而，有趣的是，观察到了一些不同的结果，并对这些结果进行了探讨。对49名血清阳性患者进行了为期两年的RF纵向研究。散射比浊法被认为比其他技术更优越，因为它能够在早期阶段检测绝对水平的变化，并且其批内变异系数较低（11%）。我们得出结论，散射比浊技术似乎适用于常规诊断用途，与更传统的方法相比具有几个优点，并且每个测试标本的成本不比罗斯-瓦勒技术高。
