Bawdon R E, Juni E, Britt E M
J Clin Microbiol. 1977 Jan;5(1):108-9. doi: 10.1128/jcm.5.1.108-109.1977.
Transformation of a Neisseria gonorrhoeae auxotroph (uracil and arginine deficient) to prototrophy was attempted with wild-type deoxyribonucleic acid from 71 random clinical N. gonorrhoeae cultures. Of these 71 cultures, 97.1% transformed the nutritionally deficient mutant to prototrophy. The procedure was reliable and economical and offered several distinct advantages over other methods used for the confirmation of N. gonorrhoeae.
尝试用来自71份随机临床淋病奈瑟菌培养物的野生型脱氧核糖核酸，将一株淋病奈瑟菌营养缺陷型菌株（尿嘧啶和精氨酸缺陷型）转化为原养型。在这71份培养物中，97.1% 将营养缺陷型突变体转化为了原养型。该方法可靠且经济，与用于确认淋病奈瑟菌的其他方法相比具有几个明显的优势。
