通过基因转化鉴定淋病奈瑟菌：一项临床实验室评估
Identification of Neisseria gonorrhoeae by genetic transformation: a clinical laboratory evaluation.
作者信息
Bawdon R E, Juni E, Britt E M
出版信息
J Clin Microbiol. 1977 Jan;5(1):108-9. doi: 10.1128/jcm.5.1.108-109.1977.
Abstract
Transformation of a Neisseria gonorrhoeae auxotroph (uracil and arginine deficient) to prototrophy was attempted with wild-type deoxyribonucleic acid from 71 random clinical N. gonorrhoeae cultures. Of these 71 cultures, 97.1% transformed the nutritionally deficient mutant to prototrophy. The procedure was reliable and economical and offered several distinct advantages over other methods used for the confirmation of N. gonorrhoeae.
摘要
尝试用来自71份随机临床淋病奈瑟菌培养物的野生型脱氧核糖核酸，将一株淋病奈瑟菌营养缺陷型菌株（尿嘧啶和精氨酸缺陷型）转化为原养型。在这71份培养物中，97.1% 将营养缺陷型突变体转化为了原养型。该方法可靠且经济，与用于确认淋病奈瑟菌的其他方法相比具有几个明显的优势。
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本文引用的文献
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J Clin Microbiol. 1976 Jul;4(1):71-81. doi: 10.1128/jcm.4.1.71-81.1976.
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