通过免疫电子显微镜检测与两起家庭肠胃炎暴发相关的26至27纳米病毒样颗粒。

Detection by immune electron microscopy of 26- to 27-nm viruslike particles associated with two family outbreaks of gastroenteritis.

作者信息

Thornhill T S, Wyatt R G, Kalica A R, Dolin R, Chanock R M, Kapikian A Z

出版信息

J Infect Dis. 1977 Jan;135(1):20-7. doi: 10.1093/infdis/135.1.20.

DOI:10.1093/infdis/135.1.20

Abstract

Viruslike particles 26-27 nm in size were detected by immune electron microscopy in stools of volunteers who were ill after administration of bacteria-free fecal filtrates derived from two separate family outbreaks of acute epidemic nonbacterial gastroenteritis. Fluorocarbon treatment and concentration of the filtrates were necessary to provide enough antigen to test sera by immune electron microscopy. Serum antibody responses were detected in both naturally occurring and experimentally induced cases of illness. The Montgomery County viruslike particle appeared to be related to the previously described Norwalk particle, whereas the Hawaii particle appeared to be unrelated to the Norwalk particle.

摘要

在接受来自两起独立的急性流行性非细菌性肠胃炎家庭疫情的无菌粪便滤液后患病的志愿者粪便中，通过免疫电子显微镜检测到了大小为26 - 27纳米的病毒样颗粒。对滤液进行氟碳处理和浓缩，对于通过免疫电子显微镜为检测血清提供足够的抗原是必要的。在自然发生和实验诱导的疾病病例中均检测到了血清抗体反应。蒙哥马利县病毒样颗粒似乎与先前描述的诺沃克颗粒有关，而夏威夷颗粒似乎与诺沃克颗粒无关。